三伏天穴位敷贴疗法对变应性鼻炎患者血清IgE、IL-4、IFN-γ的影响

摘要

Objective To establish a prokaryotio expression vector for FcγRIIb extracellular gene, to induce its expression and to purify the protein. Methods The human FcγRIIb extracellular gene was amplified by PCR and inserted into pET32a( + ).The recombinant vector pET -sFcγRIIb was analyzed by PCR,restriction enzyme digestion and DNA sequencing. The recombinant vector pET - sFcγRIIb was transformed into BL21. Through observing protein expression induced by IPTG at different temperature and time, the best induced conditionswere confirmed. The protein was detected by SDS - PAGE and then purified. Results The recombi-nant vector pET - sFcyRIIb was constructed and the husFcγRHb protein was successfully expressed after in-duced 10 hours by IPTG in SOX, and purified. Conclusion We have got the fusion protein of hus FcγRIIb by establishing pET - s FcyRIIb and laid a basis for further study of husFcγRIIb protein.%目的 观察三伏天穴位敷贴疗法对变应性鼻炎患者血清IgE及IL-4、IFN-γ的影响,为经腧穴处皮肤给药刺激经络系统达到调节免疫,治疗变应性疾病提供依据.方法 在农历三伏天对变应性鼻炎患者进行穴位敷贴治疗,采用放射免疫法检测变应性鼻炎患者治疗前后及健康体检者血清IgE、IL-4和IFN-γ.结果 治疗前变应性鼻炎患者血清总IgE及IL-4水平明显高于健康体检者(P＜0.01),血清IFN-γ与健康体检者相比差异无统计学意义(P＞0.05).经1个疗程穴位敷贴治疗后,患者血清总IgE较治疗前明显降低(P＜0.05),但IL-4和IFN-γ无明显变化(P＞0.05).结论 穴位敷贴疗法治疗变应性鼻炎可降低血清IgE水平.