高脂饮食对ApoE-/-小鼠血清生化指标的影响

摘要

Objective To investigate the changes of blood biochemical index in apolipoprotein E kockout mice fed with high-fat diet. Methods 20 six-week-male ApoE-/-mice were divided into two groups,in each group 10 mice fed with normal diet and high fat diet respectively in 24 weeks,as well as 10 C57BL/6J male mice(WT)in same age with normal diet in 24 weeks as controls. The blood samples were collected and biochemical indexes including blood lipid,myocardial enzymes and hepatic and renal function were determined with automatic biochemistry analyzer.Results Compared with the WT mice with normal diet,the levels of TG,CHOL and LDL either in ApoE-/-mice with normal diet or high fat diet were significantly increased while the level of HDL was significantly decreased (P<0.05). Compared with WT mice,the levels of AST,ALT and ALP significantly increased in ApoE-/-mice with normal diet and high fat die. The levels of CK,LDH,HBDH and ALB,CREA, UREA had no statistical significance in three groups. Conclusion The results indicated that high-fat diet increased lipdis levels in sera of ApoE-/-mice and companied with damage of liver function.%目的：探讨高脂饮食对ApoE-/-小鼠血清中血脂、心肌酶和肝、肾功能相关指标的影响及意义。方法选取6周龄雄ApoE-/-小鼠20只随机分为2组，分别给予正常饮食（ApoE-/-对照组）和高脂饮食（ApoE-/-高脂组）饲养24周；同时选取同性别、同周龄的野生型C57BL/6J（WT）雄鼠10只给予正常饮食作为正常对照组，采用全自动生化分析仪分别测定血清中血脂、心肌酶和肝肾功相关指标的变化。结果与正常对照组相比，ApoE-/-对照组血清中血脂指标甘油三酯（TG）、胆固醇（CHOL）和低密度脂蛋白胆固醇（LDL-C）明显升高，高密度脂蛋白胆固醇（HDL-C）明显降低（P均<0.05），而ApoE-/-鼠高脂组这几项指标的改变较ApoE-/-对照组也有明显差异（P均<0.05）；心肌酶学指标血清肌酸激酶（CK）、乳酸脱氢酶（LDH）和γ羟丁酸脱氨酶（HBDH）在三组间差异无统计学意义。ApoE-/-对照组和ApoE-/-高脂组的血清谷草转氨酶（AST）、谷丙转氨酶（ALT）、碱性磷酸酶（ALP）水平比正常对照组明显增高（P均<0.05），其中ApoE-/-鼠高脂组升高更明显，而白蛋白（ALB）、肌酐（CREA）和尿素氮（UREA）水平在三组间差异无统计学意义（P>0.05）。结论 ApoE-/-小鼠自发出现血清血脂指标紊乱，给予高脂饮食后进一步加重，且伴随肝功能损伤，而血清心肌酶和肾功能指标未见明显变化。