骨桥蛋白在子宫内膜样腺癌中的表达及其与肿瘤增殖和患者预后的关系

摘要

目的 探讨骨桥蛋白在子宫内膜样腺癌中的表达及临床意义.方法 采用免疫组织化学染色法检测30例正常增殖期子宫内膜组织和82例子宫内膜样腺癌组织中骨桥蛋白和反映肿瘤增殖的指标Ki-67的表达,免疫蛋白印迹进一步验证骨桥蛋白在癌组织和癌旁组织的表达.结果 免疫组织化学染色结果示骨桥蛋白在子宫内膜样腺癌组织中高表达,阳性表达率为63.4％,明显高于正常增殖期子宫内膜组织(P＜0.05),免疫蛋白印迹示骨桥蛋白在癌组织中的表达亦明显高于癌旁及正常组织.骨桥蛋白的表达与Ki-67呈正相关(r=0.393,P＜0.01).随着子宫内膜样腺癌FIGO分期、组织学分级、肌层浸润深度的增加,骨桥蛋白的表达水平也逐渐增高(P均＜0.01).Log-rank生存分析显示骨桥蛋白高表达的患者生存率较低(P＜0.05).结论 骨桥蛋白在子宫内膜样腺癌中高表达,并参与了肿瘤增殖及影响患者预后,有望成为判断肿瘤恶性程度及患者预后的新指标.%Objective To investigate the expression and clinical significance of osteopontin (OPN)in endometrioid adenocarcinoma.Methods Archived tissue samples of 30 cases of normal proliferative endometrium and 82 cases of endometrioid adenocarcinoma were selected and analyzed via IHC (immunohistochemically) with OPN as well as Ki-67,a main biologic index of tumor proliferation potentiality.Western blot was used to further validate the expression of OPN in carcinoma and adjacent tissues.Results IHC showed that OPN was overexpressed in endometrioid adenocarcinoma (63.4％),higher than that in normal proliferative endometrium,with statistical significance (P ＜ 0.05).Western blot also showedsignificantly higher OPN expression in endometrioid adenocarcinoma than in the matched adjacent tissue and normal endometrium.The expression of OPN was positively correlated with Ki-67 (r =0.393,P ＜0.01).With the increase of FIGO staging,histology grading,muscular invasion depth,the expression level of OPN gradually increased in endometrioid adenocarcinoma (all P ＜ 0.01).The higher expression of OPN was significantly associated with lower overall survival with logrank survival analysis (P ＜ 0.05).Conclusions OPN was overexpressed in endometrioid adenocarcinoma.It involved in tumor proliferation and is related with prognosis.It might be a new indicator of evaluating the malignancy and predict prognosis of endometroid carcinoma.