目的：应用三羟异黄酮对 BPH 小鼠模型和体外培养的前列腺上皮细胞进行干预治疗，观察其对 BPH 治疗作用及对细胞的影响。方法选择32只雄性昆明小鼠，应用丙酸睾丸酮肌内注射制作小鼠 BPH 模型；然后分为4组，每组各8只小鼠。其中3组小鼠分别采用10、30、90 mg／（kg ·d）三羟异黄酮剂治疗4周，余1组为对照组，不予任何治疗。4周后处死4组小鼠，取前列腺称重，计算前列腺指数。另外取 BPH 患者前列腺上皮细胞加入不同培养孔，每孔2×104个细胞，分别应用含0、0.1、0.5、1.0、2.5、5.0μmol／L 三羟异黄酮的培养基培养10 d 后收集各组细胞，染色并计数，比较6组细胞的活率。结果治疗4周后，3组实验组小鼠前列腺质量均低于对照组（P ＜0.05），中、高剂量组小鼠前列腺指数低于对照组（P ＜0.05）。应用0、0.1、0.5、1.0、2.5、5.0μmol／L 三羟异黄酮处理的前列腺上皮细胞数分别为（4.71±0.78）×104个、（4.46±1.15）×104个、（3.84±1.33）×104个、（2.01±0.31）×104个、（0.58±0.28）×104个、（0.20±0.12）×104个；台盼蓝染色提示各组前列腺上皮细胞活率分别为99.3％、98.7％、96.7％、93.3％、90.7％、88.7％，1.0、2.5、5.0μmol／L 组上皮细胞数少于对照组，细胞活率亦明显低于对照组（P ＜0.05或0.01）。结论三羟异黄酮可减轻 BPH 小鼠模型的前列腺质量，对前列腺上皮细胞增殖有抑制作用。%Objective To observe the effect of genistein on benign prostate hyperplasia (BPH)in mice and human prostatic epithelial cells (HPEC)in vitro.Methods Thirty-two Kunming mice were injected with testosterone to establish BPH models and then were divided into four groups.In three treatment groups, mice were given with different doses of genistein (1 0,30,90 mg/(kg·d))for four weeks and the mice in the control group were not treated.Then,the mice were sacrificed,the prostates were weighed and the prostate index was calculated.HPECs isolated from prostatectomy specimens of BPH patients were inoculated with cul-ture media containing 0.1 ,0.5,1 .0,2.5,5.0 μmol/L of genistein for 1 0 days,trypsinized and then counted with a hemocytometer.Results The weights of prostate in mice treated with genistein were lower than that of control group (P <0.05).Prostate indexes of middle and high dose group were lower than that of control group (P <0.05).The number of HPECs treaded with 0,0.1 ,0.5,1 .0,2.5,5.0 μmol/L of genistein was (4.71 ±0.78) ×1 04、(4.46 ±1 .1 5) ×1 04、(3.84 ±1 .33) ×1 04 ,(2.01 ±0.31 ) ×1 04 ,(0.58 ±0.28) × 1 04 and (0.20 ±0.1 2) ×1 04.Trypan blue staning revealed that the percentage of living cells was 99.3%, 98.7%,96.7%,93.3%,90.7% and 88.7%.Conclusion Genistein can suppress the growth of HEPCs and mitigate BPH induced by testosterone.
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