'靳三针'结合持续有氧运动对慢性应激r抑郁模型大鼠的干预作用

摘要

Objective To observe the therapeutic effects of Jinsanzhen acupuncture combined with continuous aerobics on the rats with chronic stress-induced depression .Methods The chronic stress-induced depression rat model was developed by using chronic unpredicted mild stimulation ( CUMS) .Thirty male SD rats were randomly divided into the normal control group , the model group ( or the experimental group ) and the treatment group .The rats in the normal control group were given normal feed , but the rats of the model group received stimulation every morning , and the rats of the treatment group also received stimulation every morning , except that they were placed on the treadmill for 30-minute running exercise every afternoon , and then, they were treated with Jinsanzhen acupuncture . Four weeks later , various indicators were detected in the rats of all the groups .Results As compared with that of the rats in the model group, the platform-jumping latency of the rats in the Jinsanzhen therapy combined with continuous aerobics group was elevated , and the number of errors was decreased (P<0.05), open field horizontal and vertical scores were all increased to certain extents (P<0.05), the latency of water maze was decreased and the number of passing through the platform were increased (P<0.05).Pathologic, elec-tron microscopic and Nissl detection results indicated that Jinsanzhen acupuncture combined with continuous aerobics could produce cer -tain effects on the brain tissue of the model group .Conclusion Jinsanzhen acupuncture combined with continuous aerobic exercise could produce good effects on the rats with chronic stress-induced depression .%目的 观察"靳三针"结合持续有氧运动对抑郁模型大鼠的作用.方法 采用慢性轻度不可预见性的刺激建立大鼠慢性应激抑郁模型(chronic unpredicted mild stress,CUMS),30只模型大鼠随机分为3组,分别为正常对照组,模型组和治疗组,每组10只.模型组每天上午给予大鼠刺激;治疗组每天上午给予大鼠刺激,每天下午将大鼠置于跑步机,进行30 min跑步训练,并使用"靳三针"疗法进行治疗;正常对照组常规喂养,4周后进行各项指标检测.结果 与模型组大鼠相比[(18.10±9.48)s、(7.00±4.78)次],"靳三针"结合有氧训练组大鼠跳台潜伏期增加[(29.50±13.43)s]、错误次数减少[(2.60±1.35)次](P<0.05);旷场水平、垂直得分均有所增加(P<0.05);水迷宫潜伏期减少、穿越站台次数增加(P<0.05);病理、电镜和尼氏结果显示"靳三针"结合有氧训练对模型大鼠脑组织有一定改善作用.结论 "靳三针"结合持续有氧运动对慢性应激诱导的大鼠抑郁具有一定的治疗作用.