目的：探讨南通地区人群细胞因子白细胞介素8(interleukin-8,IL-8)基因-251(rs4073)、+781(rs2227306)位点单核苷酸多态性(single nucleotide polymorphism, SNP)与南通地区肝细胞癌(hepatocellular carcinoma, HCC)临床表型的关系。方法：在252例 HCC 患者中，采用限制性片段长度多态性-聚合酶链式反应，(restriction fragment length polymorphism polymerase chain reaction，RFLP-PCR)方法进行IL-8-251、+781位点基因分型，分析各基因型与HCC临床表型的关联性。结果：(1)在T2~T4组中携带-251位点AT基因型的患者是携带野生基因型TT患者的2.120倍(95%CI：1.143～3.930)，携带突变基因型AT+AA者是携带野生基因型TT者的2.177倍(95%CI：1.214～3.905)。而+781位点各基因型与原发灶分期无明显关联。(2)携带-251位点突变基因型的患者出现门静脉受侵的风险较野生基因型显著增高(AT vs TT：OR=1.240，95%CI：1.044～1.473；AA vs TT：OR=1.646，95%CI：1.041～2.602；AT+AA vs TT：OR=1.293，95%CI：1.093～1.529)。携带+781位点纯合突变基因型TT者出现门静脉侵犯的风险较野生基因型CC显著增高(OR=1.571，95%CI：1.058～2.334)。(3)-251、+781位点各基因型均未发现与淋巴结转移存在明显关联。(4)-251位点各基因型未发现与HCC远处转移存在明显关联。携带+781位点纯合突变基因型TT者出现远处转移的风险较野生基因型CC显著增高(OR=1.412，95%CI：1.036～1.924)。结论：IL-8-251、+781位点多态性与南通地区HCC原发灶分期、门静脉侵犯及远处转移等临床表型存在关联。%Objective:To investigate the association between the interleukin-8(IL-8) gene-251T/A,+781C/T polymorphisms and clinical phenotypes of hepatocellular carcinoma(HCC) in Nantong area. Methods: Of 252 patients with HCC, IL-8-251 and +781 locus genotypes were genotyped by restriction fragment length polymorphism polymerase chain reaction(RFLP-PCR) and the correlation of genotypes and HCC phenotype was analyzed. Results:(1)In T2-T4 stage group, patients carrying the-251 AT genotype were 2.120 times of patients with wild genotype TT(95%CI:1.143 3.930). Who carried the mutant genotype AT+AA were 2.177 times of wild genotype TT(95%CI:1.214 3.905). Meanwhile,the genotypes of the +781 locus were not correlated with the primary tumor staging. (2)Patients carried -251 locus mutation genotypes were more likely to get the risk of portal vein invasion compared with the wild genotype(AT vs TT:OR=1.240, 95%CI:1.044-1.473;AA vs TT:OR=1.646, 95%CI:1.041 -260.2;AT+AA vs TT:OR=1.293, 95%CI:1.093-1.529). The risk of portal vein invasion in +781 locus homozygous mutant genotype TT was significantly higher than that of the wild genotype CC(95%CI:1.058, OR=1.571-2.334). (3)The genotypes of +781 and -251 locus were not associated with the metastasis of lymph nodes. (4)The genotypes of the-251 locus were not significantly associated with the distant metastasis of HCC. The risk of distant metastasis of +781 locus homozygous mutant genotype TT was significantly higher than that of the wild genotype CC(OR=1.412, 95%CI:1.036-1.924). Conclusion:The IL-8-251, +781 locus polymorphisms were associated with the primary tumor staging, portal vein invasion and distant metastasis of clinical phenotypes of HCC in Nantong area.
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