羽扇豆醇对人肝癌细胞株的作用研究

摘要

Objective To investigate the effect of lupeol on human hepatocellular carcinoma cells(HCCs). Methods SMMC7721 and HepG2 cells were treated with lupeol (25,40 and 50 μmol · L-1) ,respectively. Cell viability was observed by MTT assay. Cell growth was measured by cell cycle analysis at different time points. Cell apoptosis was detected by Annexin V/PI double staining. Results The treatment with lupeol for 48 hours significantly inhibited cell growth in SMMC7721 (IC50 = 45 μmol · L-1) and HepG2 (IC50 = 48. 5 μmol · L-1),and obviously induced cell cycle arrest in SMMC7721 with an increase in the proportion of cells in S phase from 16. 32% to 37. 19%. Furthermore, lupeol (25,40 and 50 μmol · L-1) significantly promoted cell apoptosis in SMMC7721 in a dose-dependent manner. The apoptosis rate was 5. 09% ,22. 04% and 37. 03% , respectively. Conclusion The lupeol can inhibit cell growth and induce cell apoptosis in HCCs.%目的 探讨羽扇豆醇对人肝癌细胞株的作用.方法 体外培养肝癌细胞系SMMC7721 和HepG2,用不同剂量(25、40、50 μmol·L-1)羽扇豆醇刺激,经MTT比色法观察细胞存活率,通过细胞周期测定比较羽扇豆醇刺激的SMMC7721不同时间点生长变化,Annexin V/PI双染测定不同剂量刺激下的SMMC7721凋亡率变化.结果 不同浓度的羽扇豆醇刺激SMMC7721 和HepG2 48 h后明显抑制其生长[其50%抑制浓度(IC50)分别为45 μmol·L-1(SMMC7721)和 48.5 μmol·L-1(HepG2)];羽扇豆醇刺激SMMC7721 48 h,SMMC7721细胞生长周期明显被破坏(羽扇豆醇IC50为45 μmol·L-1时刺激SMMC7721 48 h后,SMMC7721的S期由16.32%增加到37.19%);随着羽扇豆醇刺激剂量的加大,SMMC7721的凋亡率明显增加(羽扇豆醇在25、40、50 μmol·L-1时,SMMC7721的早期凋亡率分别为5.09%、22.04%、37.03%).结论 羽扇豆醇抑制人肝癌细胞株生长并促进其凋亡.