涎腺导管癌组织中EZH2、Ki-67的表达及意义

摘要

Objective To investigate the expression of EZH2 and Ki-67 proteins in salivary duct carcinoma (SDC) and normal salivary gland,(NSG), and to offer new basis for SDC's prognosis evaluation. Methods Using PV-6001 method to detect EZH2 and Ki-67's protein in 14 NSG samples and 37 SDC ones, and the experimental data were statistically analyzed through SPSS 16.0 software. Results There was statistical significance in the expression of EZH2 between SDC and NSG, as well as tumor recurrence, metastasis and diameter of tumor (P ＜0.01), but no statistical significance between gender (P>0.05). Furthermore, there was also statistical significance in the expression of Ki-67 between SDC and NSG, as well as tumor recurrence and metastasis (P＜0.0l), but no statistical significance between gender , as well as tumor size (P>0.05). Additionally, an obvious positive relationship was noted between EZH2 and Ki-67 in SDC (rs=0.849,P＜0.01). Conclusion EZH2 and Ki-67 are highly expressed in SDC, and related with tumor recurrence and metastasis. They may participate in the SDC's formation and development. The higher the coordinate expression of EZH2 and Ki-67, the more local infiltration and distant metastasis will take place. Co-detection of both EZH2 and Ki-67 is helpful to SDC's prognosis judgment.%目的 检测涎腺导管癌(salivary duct cacinoma,SDC)、涎腺正常组织(normal salivary gland,NSG)中EZH2、Ki-67蛋白的表达情况,为SDC预后评估提供可筛选的标志物.方法 应用免疫组化染色方法(PV-6001二步法)检测37例SDC、14例NSG中EZH2、Ki-67的表达,采用SPSS16.0软件对结果进行统计学分析.结果 EZH2表达在SDC与NSG、有无复发、转移、肿瘤直径之间差异有统计学意义(P＜0.01)；在患者性别中差异无统计学意义(P＞0.05)；Ki-67表达在SDC与NSG、有无复发、转移中有统计学意义(P＜0.01,P＜0.05),而在不同性别、肿瘤直径表达无统计学意义.EZH2和Ki-67在SDC中表达呈正相关(rs=0.849,P＜0.01).结论 SDC中存在EZH2、Ki-67的高表达,并与转移、复发有关,可能参与SDC的发生、发展过程,二者协同表达越高越容易发生浸润和远处转移；联合检测EZH2和Ki-67有助于SDC的预后判断.