BIRC5在非小细胞肺癌中的表达及与Tp53的关系

摘要

Objective:To investigate the expression level of the apoptotic inhibition molecule BIRC5 in non-small cell lung cancer (NSCLC) and adjacent non-cancerous tissues and its correlation with Tp53 expression and clinicopathologic parameters.Methods:Cancer versus adjacent non-cancerous tissues,formalin-fixed paraffin-embedded,of NSCLC were collected and made into tissue microarray (TMA).Immunohistochemistry (IHC) was performed to analyze the expression levels of BIRC5 and Tp53.Results:In the analysis of non-paired 139 cases of cancers versus 25 adjacent tissues,non-parametric rank test showed that BIRC5 were expressed in cancers at a higher level than that in adjacent tissues (P ＜ 0.01).Expression levels of BIRC5 were higher in male than in female patients,smokers than non-smokers patients,and squamous than non-squamous tumors (P ＜ 0.001).BIRC5 and Tp53 expressions had negative correlation (r =-0.311,P =0.011) ; Mann-Whiteney test showed BIRC5 expressed higher in patients with low levels of Tp53 than in patients with high levels of Tp53 (P ＜ 0.05).In NSCLC patients with either stages Ⅰ-Ⅳ or stages Ⅲ-Ⅳ,there was no significant difference of overall survival (OS) in patients with high levels of BIRC5 versus low levels of BIRC5.Conclusion:BIRC5 was significantly up-regulated at a higher expression level in lung cancer tissues than adjacent non-cancerous tissues and had negative correlation with Tp53 expression.The prognostic role of BIRC5 expression and its regulatory mechanisms by Tp53 warrant further investigation.%目的:探讨凋亡信号分子BIRC5在肺癌与癌旁组织中的表达差异,并分析BIRC5与Tp53和临床病理资料之间的关系.方法:制备非小细胞肺癌患者的癌与癌旁组织石蜡标本的组织芯片.采用免疫组织化学方法检测分析BIRC5、Tp53凋亡分子在组织中的蛋白表达水平.结果:BIRC5表达水平在癌组织中(n=139)较癌旁组织(n=25)显著升高(P＜0.01)；BIRC5在男性患者表达水平高于女性患者,吸烟患者中表达高于非吸烟患者,鳞癌中表达高于非鳞癌(P＜0.001).BIRC5与Tp53表达水平之间存在负相关关系(r=-0.311,P=0.011).且非参数Mann-Whiteney U检验分析显示BIRC5在Tp53高表达患者中表达高于Tp53低表达患者(P＜0.05).在Ⅰ-Ⅳ期或Ⅲ-Ⅳ期肺癌患者中,BIRC5高表达与低表达患者的OS均无统计学差异.结论:肺癌组织较癌旁组织BIRC5表达上调且与Tp53呈负相关关系.