IQGAP1在非小细胞肺癌脑转移中的作用

摘要

Objective:To study the role of IQGAP1 in brain metastasis of non - small cell lung cancer (NSCLC). Methods: Firstly to observe the different expression of IQGAP1 in NSCLC with high - potency brain metastasis PC14/ B and NSCLC with low - potency brain metastasis PC14 by Western blotting, secondly, transfer IQGAP1 to low - potency brain metastasis of NSCLC cell line PC14 and identify the expression of IQGAP1 ,then observe the migration and invasion ability after transfection. Results: The expression of IQGAP1 in NSCLC with high - potency brain metastasis was obviously higher than NSCLC with low - potency brain metastasis, the successful transfection was confirmed by RT -PCR,the ability of migration and invasion were enhanced after transferring IQGAP1 to PC14. Conclusion:Reveal IQGAP1 biological function and provide new ideas for in - depth study of lung cancer with brain metastasis.%目的:探讨IQGAP1的表达对非小细胞肺癌脑转移的影响.方法:应用Western blotting技术检测肺癌低潜力脑转移细胞与高潜力脑转移细胞中IQGAPI表达的差异；构建质粒载体pcDNA3.1-IQGAP1,将其转染至非小细胞型肺癌低潜力脑转移细胞系,并用RT-PCR方法鉴定转染效果；进而应用Transwell的方法和免疫荧光检测上调IQGAP1表达后对细胞侵袭和迁移的影响.结果:通过Western blot检测,PC14/B细胞内IQGAP1表达明显高于PC14细胞；外源性高表达IQGAP1的质粒载体pcDNA3.1-IQGAP1成功转染至PC14细胞,同时细胞形态经过转染后也发生显著变化；外源性上调的IQGAP1表达显著增强了非小细胞肺癌细胞株PC14的细胞侵袭和迁移能力.结论:IQGAP1在肺癌脑转移的过程中可能发挥了作用,为进一步研究肺癌脑转移的具体机制提供了新的思路.