强直性脊柱炎患者免疫功能的检测及其临床意义

摘要

Objective To detect the immune functions of patients with ankyiosing spondylitis(AS) to study its change and clinical significance. Methods The percentage of CD3+T,CD4+T,CD8+T,CD16+56 + NK and GD19+B lymphocytes in peripheral blood from AS and control groups were measured by flow cytometry,and the ratio of CD4+/CD8+ was calculated. Meanwhile,the levels of serum immunoglobulin IgG,IgA,IgM and complement C3,C4 were measured by nephelometry. Results The percentage of CD3+T,CD4+T,CD8+T,CD16+56+ NK,CD19+B cells and CD4+/CD8+ ratio in peripheral blood of AS group were 65. 83% ±8. 50% ,38. 33% ± 7. 21% ,24. 07% ±8. 52% , 9. 14% ±4. 90% ,15. 07% ± 6. 53% and 1. 85 ± 0. 95 respectively,and the levels of serum IgG,IgA,IgM,C3 and C4 were 14. 48 v 4. 00 g/L,3. 02 ± 1. 14 g/L,l. 53 ± 0. 92 g/L,l. 20 ± 0. 22 g/L,0. 28 ± 0. 11 g/L respectively. Compared with control groups,the percentage of CD3+T,CD4+T and CD19+B cells,the ratio of CD4+/CD8+ ,and the levels of serum IgG,IgA,IgM,C3 and C4 in AS were significantly higher ( P<0. 01 or P<0. 05) , while the percentage of CD16+ 56 + NK cells were dramatically lower (P<0. 01). There were no statistical difference of the percentage of CD8+T cells between AS patients and normal controls (PX). 05). Conclusion Cellular and humoral immunity as well as innate immunity were significantly disorder in patients with AS. Detection of the immune functions change has important clinical significance for patients with AS.%目的 检测强直性脊柱炎(ankylosing spondylitis,AS)患者的免疫功能,研究其变化及相应临床意义.方法 采用流式细胞术分别检测AS组及对照组外周血CD3+T,CD4+T,CD8+T,CD16+56+NK,CD19+B细胞占淋巴细胞的百分率,并计算CD4+/CD8+比值.免疫散射比浊法测定血清免疫球蛋白IgG,IgA,IgM以及补体C3,C4的含量.结果 AS组外周血CD3+T,CD4+T,CD8+T,CD16+56+NK,CD19+B细胞百分率及CD4+/CD8+比值分别为65.83%±8.50%,38.33%±7.21%,24.07%±8.52%,9.14%±4.90%,15.07%±6.53%和1.85±0.95,血清IgG,IgA,IgM及补体C3,C4的含量分别为14.48±4.00 g/L,3.02±1.14 g/L,1.53±0.92 g/L,1.20±0.22 g/L和0.28±0.11 g/L.其中CD3+T,CD4+T,CD19+B细胞百分率,CD4+/CD8+比值及血清IgG,IgA,IgM,C3,C4含量显著高于对照组,且差异具有统计学意义(P<0.01或P<0.05),而CD16+56+NK细胞百分率显著低于对照组(P<0.01).CD8+T细胞百分率与正常对照组差异无统计学意义(P>0.05).结论 AS患者存在细胞免疫、体液免疫以及天然免疫紊乱.检测AS患者免疫功能的变化具有重要临床意义.