目的 分析不同pH、贮存温度及放置时间对测定尿β2-微球蛋白(β2-MG)结果的影响并提出纠正方法.方法 210例共420份尿液标本(每例2份)以尿常规pH值分成7组(pH4.5,pH5.0,pH5.5,pH6.0,pH6.5,pH7.0,pH7.5,每组30例,含肾小管损伤的病例组20例,健康对照组10例),在不同pH、贮存温度(25℃,4℃)及放置时间(0,60,120,240 min)采用Axsym免疫分析仪分别测定每份标本的尿β2-MG结果,分析不同影响因素对测定结果的干扰程度并进行校正.结果 相同贮存温度及放置时间各pH组内病例组与对照组之间的尿β2-MG降解率差异无统计学意义(t=0.156~1.852,P>0.05);25℃时pH5.0以下,4℃时pH4.5的尿β2-MG降解率、测定值与贮存温度、放置时间存在差异且与放置时间呈负相关(t=2.486~21.685,P<0.05或<0.001;相关系数r=-0.863~-0.946,P<0.05),采用校正系数对尿β2-MG测定结果进行校正,平均相对误差在1.99%~8.58%之间.结论 对25℃时pH5.0以下、4℃时pH4.5的尿β2-MG测定结果进行校正是可行的和可靠的.%Objective To explore the influence of different urinary pH,preserving temperature and storage time on β2 micro globulin (β2 MG) determination in urine,and put forward the correcting method. Methods According to routine analysis re suits of urinary pH,210 cases were divided into 7 groups with urinary pH range of 4. 5 to 7. 5,including 20 patients with re nal tubular injury (case group) and 10 health individuals (control group) in each group. The results of β2 MG in each of 420 urine specimens were measured by the Axsym analyzer at different urinary pH, preservating temperature (25℃ ,4℃) and storage time (0,60,120,240 min) , respectively. Effect of different factors on results of β2 MG determination were analyzed and corrected. Results The degradation rate of urinary β2 MG showed no significant difference between the case and the control group in each pH group at same preservating temperature and storage time (t= 0. 156~1. 852, P>0. 05). There were differences between degradation rate or the value of β2 MG determination and preservating temperature or storage time (t=2. 486 ~ 21. 685, P<0. 05 or <0. 001) ,and it were negatively correlated with the storage time (r= - 0. 863~-0. 946,P<0. 05 ) ,and the average relative errors were 1. 99‰~8. 58‰ when the results of urinary β2 MG determination were fur ther corrected with the correction coefficient,in urine specimens below pH5. 0 groups at 25℃ and pH4. 5 group at 4℃. Conclusion It is feasible and reliable for correction of urine β2 MG results.
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