血管生成素样蛋白1和2水平在胃癌患者中的表达及临床意义

摘要

目的：探讨血管生成素样蛋白（Ang）1和2水平在胃癌患者中的表达以及临床应用价值。方法收集2012年6月～2014年12月胃癌患者65例，选择同期健康体检者50例为对照组，采用酶联免疫吸附试验（ELISA 法）检测研究对象中 Ang-1和 Ang-2水平，并与对照组作比较。结果胃癌组中 Ang-1水平（19.8±2.1μg／L）和对照组（19.5±1.7μg／L）间差异无统计学意义（P ＞0.05），Ang-2水平（2.3±0.8μg／L）显著高于正常对照组（0.8±0.2μg／L，t ＝2.50，P ＜0.01）；不同胃癌病理分型中 Ang-2水平差异无统计学显著性意义（P ＞0.05）。Ⅲ～Ⅳ期患者的 Ang-2水平（2.6±0.5μg／L）显著高于Ⅰ～Ⅱ期（1.6±0.4μg／L）患者，有淋巴结转移的患者 Ang-2水平（2.7±0.5μg／L）显著高于无淋巴结转移（1.6±0.5μg／L）的患者，而且术后1年复发患者的 Ang-2水平（2.0±0.6μg／L）显著高于未复发患者（1.2±0.5μg／L），经比较差异均有统计学意义（P 均＜0.05）。Ang-2水平和肿瘤的分期和淋巴结转移均呈正相关（r 分别为0.31和 0.33，P 均＜0.05）。结论胃癌患者中 Ang-2水平显著升高，与肿瘤分期、转移和预后相关，对胃癌病程进展、疗效观察和预后评估有重要的临床价值。%Objective To explore the expressions and clinical value of angiopoietin like protein 1 (Ang-1)and angiopoietin like protein 2 (Ang-2)in patients with gastric cancer.Methods 65 patients with gastric cancer and 50 healthy subjects for con-trol group were collected from June in 2012 to December in 2014.The level of Ang-1 and Ang-2 were determined by enzyme-linked immunosorbent assay (ELISA).The results were compared.Results Compared with control group (19.8±2.1 μg/L),the level of Ang-1 in patients with gastric cancer (19.8±2.1 μg/L)was no statistics difference (P >0.05),but Ang-2 (2.3±0.8 μg/L)was significantly higher than those of healthy subjects (0.8±0.2 μg/L,t=2.50,P <0.01).There was no significant difference of Ang-2 level in different pathological types of gastric cancer (P >0.05).The level of Ang-2 in TNM stage Ⅲ~Ⅳ (2.6±0.5 μg/L)was obviously higher than that in stage.Ⅰ~Ⅱ (1.6±0.4 μg/L).Ang-2 levels were signif-icantly higher in patients with lymph node metastasis (2.7±0.5 μg/L)or postoperative recurrence after one year (2.0±0.6μg/L)than those without lymph node metastasis (1.6 ± 0.5 μg/L)or postoperative recurrence (1.2 ± 0.5 μg/L,P <0.05).The level of Ang-2 in gastric cancer was significantly positive correlated with tumor stage and lymph node metastasis (r=0.31 and 0.33 respectively,P <0.01).Conclusion Ang-2 level was significantly increased in gastric cancer.Its level was significantly correlated with tumor stage,lymph node metastasis and prognosis,which has important application value for the cancer progression,clinical observation and prognosis evaluation of gastric cancer.