锰超氧化物歧化酶9 Ala／Val 基因多态性对冠心病的影响

摘要

Objective To study associations between manganese superoxide dismutase 9 Ala/Val (Mn-SOD 9 Ala/Val)genet-ic polymorphism and total superoxide dismutase (T-SOD)and Mn-SOD activity and the impact on coronary heart disease (CHD)were studied.Methods There were 82 CHD patients and 57 controls in this research.Sequencer was used to identify the genotype of Mn-SOD 9 Ala/Val genetic polymorphism and colorimeter was used to detect the serum T-SOD and Mn-SOD activity.Results Compared with the control group,the serum T-SOD and Mn-SOD activity of the CHD group was significantly reduced(t=4.83,6.57,P all<0.05),while the VV genotype and V allele of Mn-SOD 9 Ala/Val genetic poly-morphism of the CHD group were higher (χ2 =4.75,P <0.05).The serum T-SOD and Mn-SOD activity of the Mn-SOD 9 VV genotype was significantly lower than the Mn-SOD 9 AA genotype(t=2.96,3.11,P all<0.05).Conclusion The ser-um T-SOD and Mn-SOD activity in the CHD patients was reduced.Mn-SOD 9 Ala/Val genetic polymorphism was involved in the pathogenesis of CHD by influencing the Mn-SOD activity.%目的：研究锰超氧化物歧化酶（Mn-SOD）9 Ala／Val 基因多态性与 T-SOD 和 Mn-SOD 活性的关系以及对冠心病（CHD）的影响。方法共82例冠心病患者和57例正常人参与研究，应用测序法检测两组的 Mn-SOD 9 Ala／Val 基因多态性的基因型，应用比色法检测两组的血清 T-SOD 和 Mn-SOD 活性。结果冠心病组的血清 T-SOD 和 Mn-SOD 活性低于对照组（t＝4.83，6.57，P 均＜0.05），冠心病组的 Mn-SOD 9 VV 基因型和 V 等位基因频率高于对照组（χ2＝4.75，P ＜0.05），Mn-SOD 9 VV 基因型的血清 T-SOD 和 Mn-SOD 活性低于 Mn-SOD 9 AA 基因型（t＝2.96，3.11，P 均＜0.05）。结论冠心病患者的血清 T-SOD 和 Mn-SOD 活性降低，Mn-SOD 9 Ala／Val 基因多态性通过影响 Mn-SOD 的活性进而参与冠心病的发病。