核不均一性核糖核蛋白H2（hnRNPH2）对单纯疱疹病毒（HSVI）复制影响

摘要

本文研究了单纯疱疹病毒（HSVI）在感染与复制过程中影响宿主细胞RNA转录，蛋白表达，进而为病毒的潜伏和持续感染创造必要条件．采用基于2-DE分析，人正常肝细胞L-02细胞核不均一性核糖核蛋白H2（heterogeneous nutlear ribonucleoprotein H2，hnRNPH2）在感染HSVI前后存在表达差异，构建pcDNA-hnRNPH2真核表达载体及hnRNPH2稳定转染的293T细胞系的方法，研究在核不均一性核糖核蛋白H2（hnRNPH2）稳定过表达后对HSVI病毒复制周期的影响．结果表明，病毒滴度和生长曲线显示，hnRNPH2可以显著影响病毒复制周期．过表达hnRNPh2的细胞系与对照相比，其病毒滴度明显下降，hnRNPH2在HSVI病毒复制中起到比较明显的抑制作用，其作用机理还有待进一步研究．%Herpes simplex virus I （nsv I ） regulating the pathway modify in host cell is a very systematic and complicate system. of transcription and translation By the analysis of 2-DE, the heterogeneous nuclear ribonucleoprotein H2 in human fetal liver cell represent distinction after the HSV I infection. Establish the stable transfection hnRNP H2 cell line. Analysis post infection of different cell lines, the one-step growth curve and titer of HSV I have changed dramatically. Accordingly, modulation hnRNP H2 during infections such as HSV may change the replicative cycle