SN38对Fas介导凋亡效应的影响

摘要

In order to investigate the mutual relationship between SN38 and Fas-mediated apoptosis and to further study the role of lipid raft (abundant in sphingomyelin) in the process, in the experiments SN38 and CH11 were worked out to react independently or jointly on lymphoma cell strains of lipid raft positive and lipid raft negative WR/ Fas-SM( - ) cells and WR/Fas-SMSl cells, and observe the effect differences whether the SN38 could enhance Fas-mediated apoptosis and two cells through flow cytometry, DNA ladder and other experimental methods. The results showed joint application of SN38 and CH11 to induce more noticeable apoptosis to happen in WR/Fas-SMSl cells than in WR/Fas-SM( - ) cells as compared with application of SN38 alone. Yet the enhancement on WR/Fas-SMS( - ) cells apoptosis was not noticeable, prompted that lipid raft played a certain effect on the enhancement of SN38 during the process of WR/Fas-SMSl cell Fas mediated-apoptosis.%为探讨SN38与Fas介导凋亡的相互关系,并进一步探讨脂筏(富含鞘磷脂)在该过程的作用,本实验拟单独或者联合应用SN38和CH11作用于脂筏阴性和脂筏阳性的淋巴瘤细胞株WR/Fas-SM(-)细胞和WR/Fas-SMS1细胞,通过流式细胞术、DNA ladder等实验方法观察SN38能否促进Fas介导的凋亡以及对2细胞的效应差异.结果显示,SN38与CH11联合应用与单独应用SN38相比,可以诱导WR/Fas-SMS1细胞更加明显凋亡现象的发生,但对WR/Fas-SM(-)细胞凋亡的促进作用不明显,提示脂筏在SN38促进WR/Fas-SMS1细胞Fas介导凋亡过程中起到一定作用.