兰州市空气微生物群落的碳代谢特征及功能多样性研究

摘要

应用Biolog ECO技术和RDA分析研究了兰州市不同功能区,即风景区(Scenic Area,SA)、交通干线(Main Traffic Line,MTL)、公共服务区(Public Service Area,PSA)、文教区(Culture and Education Area,CEA)空气微生物群落代谢功能多样性及其与环境因子的相关性.结果表明,不同功能区的AWCD值在培养192 h后进入稳定期,对碳源总体利用能力呈现显著的区域性差异,SA>PSA>CEA≈MTL.受环境因素影响,McIn-tosh指数差异明显,SA和PSA物种均一度较高.兰州市空气微生物相对利用率较高的碳源类型是酯类、氨基酸类及醇类,与主成分1显著相关的碳源有10种,其中氨基酸类是导致兰州市空气微生物群落代谢功能区域性差异的主要分异类型.环境因子与空气微生物群落代谢功能密切相关,温度、风速是不同功能区存在差异的主导因子.%The diversity of metabolic function of airborne microbial communities and their reciprocity with environ-mental factors at different functional regions (Scenic Area(SA),Main Traffic Line (MTL),Public Service Area (PSA),Culture and Education Area (CEA))in Lanzhou city were studied using Biolog ECO technique and redun-dancy analysis (RDA)method. The results showed that the average well color development (AWCD)values of differ-ent functional regions entered into stationary phase after cultivation for 192 h,and showed significant regional differ-ences in the overall utilization of carbon source,SA > PSA > CEA≈MTL. Caused by the influence of environmental factors,McIntosh indexes in different regions were significantly different. And SA and PSA species were all compara-tively greater for a time. The mainly used utilization of carbon sources by airborne microbes in Lanzhou city are esters, amino acids,and alcohols. There are 10 types of carbon sources that are significantly correlated to principal compo-nent 1,among them amino acids are the main differentiation category that caused the regional difference in metabolic function by airborne microbes in Lanzhou city. Environmental factors are closely related to metabolic function of air-borne microbial communities,temperature and wind speed were the magistral factors that caused the difference existed at different functional regions.