斑马鱼作为一种脊椎动物模型，是研究病原菌感染与免疫的理想对象，目前已成功构建了多种细菌感染斑马鱼模型。由于其在通体透明的幼鱼阶段只有天然免疫系统发育完善，因此利用斑马鱼研究天然免疫具有许多优势，且能避免获得性免疫的干扰。自噬作为一种高度保守的胞内降解过程，是天然免疫的重要部分，可通过Toll样受体或黏膜免疫系统等参与对细菌的应答。目前，对斑马鱼自身发育需要及清除母体残留物所进行的自噬已进行了初步研究。最近细菌感染斑马鱼的体内自噬研究模型已成功构建，这对体内异源自噬机制的研究意义重大。本文就近年来利用斑马鱼模型研究病原菌感染与免疫及自噬的进展作一综述。%As a well-known vertebrate model ,zebrafish is an ideal animal model to study infection and immu-nity .Many bacteria-zebrafish infection models have been constructed successfully .On its translucent larval stage ,only innate immune system has developed perfectly .So using zebrafish to study innate immunity has many advantages and can avoid the interference of adaptive immunity .As a highly conservative intracellular degradation process ,autophagy is an important event of innate immunity .It can initiate a response against bacteria through Toll-like receptors or mucosal immune system .A preliminary study on autophagy of ze-brafish associated with its development and eliminating maternal residues has been going on .And now a new bacteria-zebrafish model for studying autophagy has been constructed successfully ,which is of great signifi-cance to study the mechanisms of xenophagy . Here we summarize the results gained from zebrafish on pathogen infection and immunity as well as autophagy in recent years .
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