Doping Gd^(3+)Ion in PDA-PHBV Coating on Ti6Al4V Alloy for Enhancing Corrosion Resistance and Proliferation of Human Gingival Fibroblasts and Human Umbilical Vein Endothelial Cells

摘要

In this study,we fabricated poly(3-hydroxybutyrate-3-hydroxyvalerate)(PHBV)coatings doped with Gd^(3+)(1,5,and 10×10^(−4) mol/L)on Ti6Al4V alloy for the first time to promote soft tissue sealing around dental implants.The corrosion resistance of Gd^(3+)-modified PHBV-coated Ti6Al4V was studied by electrochemical and immersion tests,respectively,whereas CCK-8 and RT-PCR evaluated the biocompatibility to human gingival fibroblasts(HGFs)and human umbilical vein endothelial cells(HUVECs).It was found that the Gd^(3+)-modified PHBV coating could enhance the corrosion resistance of Ti6Al4V.In vitro cell tests showed that PHBV coatings with and without Gd^(3+) addition could promote adhesion and proliferation of HGFs and HUVECs,showing a Gd^(3+) content-dependent manner.Moreover,it was found that the PDA-PHBV@1Gd showed the best proliferation to HGFs by up-regulating gene expressions of VINCULIN,ITGB1,and ITGA3,whereas the best response to HUVECs with the highest gene expression of eNOS and HIF-1αgenes was found in the PDA-PHBV@5Gd-coated group.