精子 DNA 损伤对试管婴儿助孕结局的影响

摘要

Objective:Detection of DNA damage of sperm,the assessment of the sperm quality and then explore the in-fluence of sperm DNA damage on test tube baby help pregnant outcome.Methods:We collect 68 patients who turn to help for test tube baby assisted reproductive technology from January 201 1 to December 2014 in our hospital.The 68 patients were in accordance with the relevant provisions which the ministry of health on the revision of 《human assisted reproductive technology and human sperm bank relevant technical standards,basic standards and ethical principles》,for men,there are not serve oligozoospermia or obstructive azoospermia.According to the DNA damage of sperm into sperm DNA damage group(sperm DNA damage rate ≥ 30%)and normal group (sperm DNA damage rate < 30%). Through a variety of means testing,get the acrosin activity of the 68 cases of male sperm as well as the sperm concen-tration,sperm activity,invitro fertilization rate,the percentage of normal sperm morphology,good quality embryo rate and live birth rate,deformity rate,pregnancy outcome and other indexes of results.Then observe and compare the a-bove indexes between the two groups.Results:The test results of injury group were less than the normal group.The a-bortion rate of the normal group compared to the abortion of the injury group does not have statistical significance. Conclusion:The integrity of the sperm will affect the fertilization ability,so the detection outcome of the sperm DNA damage rate of test tube baby help pregnancy success rate has certain influence.%目的：：检测精子 DNA 损伤情况，评估精子的质量，进而探究精子 DNA 损伤对试管婴儿助孕结局的影响。方法：以2011年1月—2014年12月在我院进行试管婴儿辅助生殖技术的其中68例患者为例，均符合《卫生部关于修订人类辅助生殖技术与人类精子库相关技术规范、基本标准和伦理原则的通知》的相关规定，男性均不存在严重少精症或梗阻性无精子症。根据男方精子的 DNA 损伤情况分为精子 DNA 损伤组(精子 DNA 损伤率≥30%)和正常组(精子 DNA 损伤率<30%)。通过各种检测手段得出68例男性精子的顶体酶活性、精子浓度、精子活性、体外受精率、正常形态精子百分率、优质胚胎率及活产率、畸形率、妊娠结局等各项指标结果，观察比较两组病例的以上指标情况。结果：损伤组的各项指标测定结果均小于正常组，正常组的流产率与损伤组的流产率相比不具备统计学意义。结论：精子DNA 完整性影响精子的受精能力，因而精子 DNA 损伤率的检测结局对试管婴儿的助孕成功率具有一定的影响。