三七对酒精性肝病大鼠肝脏细胞因子的影响

摘要

目的 观察三七对酒精性肝病大鼠肝组织TNF-α、Leptin、IL-6、IL-8等因子的影响,探讨其抗酒精性肝病的作用机制.方法 70只雄性SD大鼠随机分为模型组、三七低剂量组、三七高剂量组和硫普罗宁组各15只及正常组10只,模型组大鼠灌服白酒-玉米油-吡唑混合液14周建立酒精性肝病模型,三七低、高剂量组和硫普罗宁组大鼠在造模的同时予以相应的药物干预,正常组以蒸馏水代替,连续14周;酶联免疫法和放射免疫法检测肝组织中TNF-α、Leptin、IL-6及IL-8等细胞因子的含量.结果 模型组大鼠肝组织TNF-α、Leptin、IL-6及IL-8含量较正常组明显升高(P<0.01),应用不同剂量三七及硫普罗宁干预后肝组织TNF-α、Leptin、IL-6及L-8含量较模型组明显降低(P<0.05).结论 三七防治大鼠酒精性肝病的机制可能与降低ALD大鼠肝组织TNF-α、Leptin、IL-6及IL-8等细胞因子的水平有关.%Objective To evaluate the effect of Sanchi on expression of hepatic TNF - α , IL - 6 , IL - 8 and leptin in rats with alcoholic liver disease ( ALD) . thus to explore the mechanism of Sanchi in alcoholic liver disease. Methods Seventy SD rats were randomly divided into five groups: the normal group ( n = 10) , the model group ( n = 15 ) , the high - dose Sanchi group ( n = 15 ) , the low - dose Sanchi group ( n = 15 ) , and the tiop ronin group ( n = 15 ) . Alcoholic liver disease in rats of the model group waa induced with mixture of alcohol - corn oil - pyrazole in 14weeks. Rats in the high - dose Sanchi, the low - dose Sanchi, and the tiop ronin group were treated with different medicine. The contents of hepatic TNF -α,IL -6,IL -8 and leptin were examined with ELISA or RIA. Results In the model group, the amounta of hepatic TNF - α , IL - 6 , IL - 8 and leptin were higher than tbose in the normal group( P ＜0.01 ) , and all those in the treatment groups were lower than those in the model group ( P ＜ 0. 05 ) . Conclusion Sanchi can markedly reduce the levels of hepatic TNF - α, IL - 6 , IL - 8 and leptin. This may serve as one of the critical mechanisms through which Sanchi efficiently prevents from alcoholic liver disease.