CXCR4和MMP-9在胃癌组织中表达及其意义

摘要

Objective To investigate the relationship between the expression of CXCR4 and MMP -9 in gastric cancer and several clinicopathological factors. Methods A total of 76 cases of gastric cancer were enrolled in this study. Immunohistochemical staining was performed to detect the expression of CXCR4 and MMP -9 in gastric cancer. And statistical analysis technique was used to investigate the relationship between CXCR4 and MMP-9 expressions and the clinicopathological factors. Results The total positive rate of CXCR4 expression in these samples was SS. 26% , but there was no positive expression in the normal stomach tissue. The positive expression of CX-CR4 was higher in these cases with lymphnode metastasis than that of patients without lymphnode metastasis (73.91% vs 36.37% , P = 0. 000). The positive rate of CXCR4 expression in these cases with peritoneal metastasis was higher than that of patients without peritoneal metastasis ( 100% vs 39.28% , f<0.000). Meanwhile, the positive rate of MMP -9 expression in normal stomach tissue and gastric cancer tissues were IS. 79% and 67. 11% , respectively. The positive expression of MMP -9 was higher in these cases with lymph node metastasis than that of patients without lymph node metastasis (80.43% vs 46.67% , P =0.002). Furthermore, the positive expression of MMP -9 with peritoneal transfer group was higher than that of patients without peritoneal transfer group ( 100% vs 55. 36% , P = 0. 000) . The expression level of CXCR4 and MMP - 9 were closely correlated with histological cellular differentiation, invasion depth ( P = 0.000, 0.001, 0.002, 0.013) , and the positive expression of CXCR4 was consistent with the positive expression of MMP -9 (r = 0. 406,P = 0. 000). Conclusion Expressions of CXCR4 and MMP -9 are closely related to the degree of malignancy of gastric cancer, which may provide an evidence for diagnosis and assessment of prognosis in gastric cancer.%目的 探讨趋化因子受体4(CXCR4)和基质金属蛋白酶-9(MMP-9)在胃癌中的表达和临床意义.方法 应用S-P免疫组织化学方法检测CXCR4和MMP-9在76例胃癌组织及癌旁正常胃组织中的表达水平,并分析其与胃癌临床病理因素之间的相关性.结果 CXCR4在癌旁正常胃组织中无阳性表达,在胃癌组织中的阳性表达率为55.26％；CXCR4在伴有淋巴结转移与无淋巴结转移的胃癌组织中阳性表达率分别为73.91％和36.37％,差异有统计学意义(P=0.000)；CXCR4在伴有腹膜转移的胃癌组织中阳性表达表达率为100％,显著高于无腹膜转移组(39.28％,P=0.000),且同肿瘤的分化程度及浸润深度关系密切(P分别为0.000、0.001).MMP-9在胃癌组织中阳性表达率(67.11％)明显高于癌旁正常胃组织(15.79％)(P＜0.000)；MMP-9的在胃癌中的阳性表达与肿瘤组织分化程度、浸润深度、伴淋巴结转移及腹膜转移有关(P分别为0.002、0.013、0.002、0.000).CXCR4与MMP-9的阳性表达呈正相关(r=0.406,P=0.000).结论 CXCR4和MMP-9表达强度与胃癌的恶性程度密切相关.