Objective To assess whether the level of serum soluble lectin-like oxidized low-density lipoprotein receptor-1 (sLox -1) is associated with LOX-1 gene polymorphism.Methods Levels of sLox-1 were measured in 345 patients of Han population of eastern area of Zhejiang Province who come to our centre seeking for coronary angiography with enzyme-linked immunosorbent assay,including 45 acute myocardial infarction patients,86 unstable angina pectoris patients,89 stable angina pectoris patients and 136 noncoronary patients excluded by coronary arteriography.The polymorphisms of gene LOX-1 G501C and 3 'UTR-C/T were measured by the way High-throughput SNP genotyping with single-tube PCR with Tm-shift primers.Results The frequencies for the 3'UTR-C/T TT,TC,CC were 5.4％,37.3％,57.3％,and G501C CC,GC,GG were 5.4％,31.9％,62.7％,respectively.The level of sLox1 were 160.0 ±56.1,132.7 ±52.0,128.9 ±58.0pg/ml in 3'UTR-C/T TT,TC,CC group,and 134.0 ±55.2,127.2 ±50.8,134.2± 58.7pg/ml in 501G/C CC,GC,GG group.There were no discrepancy in plasma sLOX-1 levels between different groups.In subgroup analysis,there were no discrepancy too.Conclusion Levels of sLox-1 were not associated with the polymorphisms of gene LOX -1 in Han population of eastern area of Zhejiang Province.%目的 观察血浆中sLOX-1浓度是否和LOX-1基因多态性相关.方法 采用酶联免疫吸附法测定354例接受冠状动脉造影的浙东地区汉族人群住院患者血清中sLOX-1的浓度,并采用Tm-shift基因分型方法对G501C和3'UTR-C/T等位基因型进行检测,其中包括43例急性心肌梗死、86例不稳定型心绞痛、89例稳定型心绞痛和136例经冠状动脉造影排除冠心病的患者.结果 3 'UTR-C/T位点TT,TC,CC基因型分别为5.4％,37.3％,57.3％,501G/C位点CC,GC,GG基因型分别为5.4％,31.9％,62.7％,3'UTR-C/T TT,TC,CC 3组sLOX-1浓度分别为160.0 ±56.1,132.7±52.0,128.9±58.0pg/ml,501G/C CC,GC,GG 3组sLOX-1浓度分别为134.0±55.2,127.2±50.8,134.2士58.7pg/ml.不同基因型患者sLOX-1浓度各组间没有显著性差异,在各亚组间也没有差异.结论 浙东地区汉族人群sLOX-1浓度和LOX-1基因多态性无相关性.
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