肝内胆管结石与Apo AⅠ-CⅢ-AⅣ基因PstⅠ位点多态性的关系研究

摘要

Objective To study the relationship between Apolipoprotein A Ⅰ 、CⅢ 、AⅣ gene cluster polymorphism at Pst Ⅰ and the happening of hepatolithiasis.Methods Totally 64 hepatolithiasis patients and 120 healthy controls in Zhejiang Province were studied.Restriction fragment length polymorphisms(RFLP) at PstI、SstI sites of Apo AⅠ-CⅢ-AⅣ gene cluster were studied using polymerase chain reaction (PCR).Results The genotype frequency of A1 A1,A1 A2,A2A2 in the hepatolithiasis group and control group was separately 0,4.7％,95.3％ and 0,10.0％,90.0％.The genotype frequency and gene frequency both were of no difference between hepatolithiasis group and control group (P ＞ 0.05).Conclusion The data showed that PstI RFLP of ApoAⅠ-CⅢ-AⅣ gene cluster is not associated with hepatolithiasis in Chinese patients.%目的 探讨载脂蛋白AⅠ-CⅢ-AⅣ(apolipoprotein AⅠ-CⅢ-AⅣ,Apo A Ⅰ-CⅢ-AⅣ)基因PstⅠ位点多态性与肝内胆管结石易感性(the happening of hepatolithiasis)的关系.方法 应用PCR和限制性内切酶技术对64例肝内胆管结石患者和120例正常人Apo A Ⅰ-CⅢ-AⅣ基因Pst Ⅰ位点进行限制性片段长度多态性(restriction fragment length polymorphisms,RFLP)分析.结果 肝内胆管结石组中A1A1、A1A2、A2A2基因型频率分别为0、4.7％、95.3％,正常对照组中A1A1、A1A2、A2A2基因型频率分别为0、10.0％、90.0％.肝内胆管结石组和正常对照组各基因型频率的比较(x2=1.599,P=0.206)和等位基因频率的比较(x2=1.531,P=0.216)差异均无统计学意义.结论 Apo A Ⅰ-CⅢ-AⅣ基因Pst Ⅰ位点多态性可能不是肝内胆管结石的危险因素.