香鳞毛蕨对脂多糖诱导的人真皮微血管内皮细胞增殖及mRNA表达的影响

摘要

目的 研究香鳞毛蕨醇提物对脂多糖(lipopolysaccharides,LPS)诱导的人真皮微血管内皮细胞(human dermal microvascular endothelial cells,HDMEC)炎性反应的影响.方法 分别用0、0.4、2和10μg/ml的LPS作用于人真皮微血管内皮细胞,孵育24h建立微血管内皮细胞炎性模型.实验分为HDMEC空白对照组、LPS模型组和LPS+香鳞毛蕨药物组,分别进行药物处理,观察不同剂量香鳞毛蕨醇提物处理对各组HDMEC细胞增殖和炎症相关基因mRNA表达的影响.结果 香鳞毛蕨醇提物可以抑制2μg/ml LPS刺激下的微血管内皮细胞的增殖(P＜0.05),并降低脂多糖所诱升的血管内皮生长因子VEGF以及炎症相关基因NF-κB、VCAM-1的表达水平(P＜0.01).结论 香鳞毛蕨醇提物可能通过抑制炎症相关基因的表达来抑制微血管内皮细胞的炎性增殖.%Objective To study the anti-inflammation effect of Dryopteris fragrans (L.) Schott.extract(Herbs,in brief) on lipopolysaccharides (LPS) treated dermal microvascular endothelial cells.Methods Human dermal microvascular endothelial cells(HDMECs) were divided into control group;LPS group,which were treated with 0.4,2 and 10μ g/ml LPS for 24 hours,respectively;and LPS + Herbs group,which were pre-treated with 10 times diluted Dryopteris fragrans (0,0.1 and 1mg/ml,respectively) for 1 hour,then treated with LPS for 24 hours,respectively.Changes of cell proliferation and expression in inflammatory associated genes in each group were detected.Results Dryopteris fragrans inhibited proliferation of HDMEC induced by 2μg/ml LPS(P ＜ 0.05),accompanied with down-regulation of VEGF gene expression (P ＜ 0.01).The expression of NF-κB,VCAM-1 also decreased in LPS treated HDMEC when the cells were pre-treated with Dryopteris fragrans (P ＜ 0.01).Conclusion Dryopteris fragrans (L.) Schott.extract may inhibit LPS induced inflammatory reaction of microvascular endothelial cells by regulating the expression of inflammation associated genes.