亚甲基四氢叶酸还原酶C677T基因多态性与2型糖尿病关系的病例对照研究

摘要

目的 探讨亚甲基四氢叶酸还原酶(MTHFR)基因C677T多态性与2型糖尿病(T2DM)的关系.方法 选取226例T2DM患者(病例组)和194例健康对照个体(对照组),检测两组同型半胱氨酸水平,采用PCR-芯片杂交技术检测亚甲基四氢叶酸还原酶C677T多态位点基因型,计算等位基因频率并统计分析.结果 2组MTHFR基因C677T位点基因型和等位基因频率比较,差异均无统计学意义(P＞0.05),MTHFR基因TT型同型半胱氨酸水平显著高于CC型及CT型.结论 MTHFR基因C677T多态性影响同型半胱氨酸水平,但与T2DM的发病无明显的相关性.%Objective To examine the relationship between methylenetetrahydrofolate reductase (MTHFR) gene C677T polymorphism and type 2 diabetes mellitus(T2DM).Methods MTHFR gene C677T polymorphic sites were assayed in 226 T2DM and 194 healthy subjects by PCR-DNA microarray.Homosysteine levels were measured.And their genotype and allele frequency were statistically analyzed.Results There was no statistical significance in allele frequency and genotype of MTHFR gene C677T between the patients and healthy subjects(P ＞ 0.05).The homosysteine levels were obviously higher in TT genotype.Conclusion MTHFR gene C677T polymorphism is not a risk factor for patients with type 2 diabetes,but can effect homosysteine levels.