植物差异蛋白组学研究中几项主要技术的探讨

摘要

蛋白质是细胞内的关键物质，是生命活动的体现者。对蛋白质的研究，将有助于阐明生命现象的某些重要机制。目前植物高通量差异蛋白组学技术的发展日新月异，但主要包括双向电泳（2-DE ）、双向荧光差异凝胶电泳（DIGE）、同位素亲和标签（ICAT）、同重同位素标记相对和绝对定量（iTRAQ）4种技术方法。因此，就以上4种技术方法进行探讨，以期更好他利用蛋白组学相关技术。%Protein is the key material in the cell and the agent of life activity.The study of protein will help clarify some important mechanism of life phenomenon.At present,the plant high-throughput differential proteomics has developed quickly,but it mainly in-cludes four technical methods,two-dimensional electrophoresis (2-DE),Differential In-Ge1 Electrophoresis (DIGE),Isotope Coded Affinity Tag (ICAT)and with heavy isotope labeling relative and absolute quantitative (iTRAQ).In this paper,we discussed the above four technical methods in order to better use proteomics technology.