Ionomycin诱导小鼠精子顶体反应的初步研究

摘要

Aim:To investigate the spermmatozoa acrosome-reaction (AR)induced by ionomycin. Methods:Motile spermatozoa of the mouse cauda epididymides were treated with ionomycin at different concentrations for different times.Sperm acrosomal status was detected by PNA-FITC staining.Results:Spermatozoa acrosome-reaction was observed after ionomycin treatment.The percentages of acrosome-re-acted spermatozoa were 31.6%,52.2%,57.4%and 64.0%after 1 hour-induction by ionomycin at the final concentration of 1 ,5,1 0,50 μmol/L respectively.When the spermatozoa were treated with 5 μmol/L.Ionomycin for 1 0,30,45,60,1 20,1 80 minutes,the percentages of AR were 1 7.2%,25.0%, 41.2%,52.2%,54.0%,55.0% respectively.Conclusion:Mouse sperm AR has been induced by ionomycin treatment.the percentage of AR sperm varied with the concentration of ionomycin and incuba-tion time.%目的：探讨Ionomycin诱导精子顶体反应的效果．方法：收集小鼠附睾尾精子，体外获能后用不同终浓度的Ionomycin处理相同时间，以及用相同浓度的Ionomycin处理不同时间．FITC-PNA染色观察并统计精子顶体反应率．结果：用终浓度为1、5、10、50μmol/L Ionomycin 处理1 h 的精子 AR 率分别为31.6％、52.2％、57.4％和64.0％．用终浓度为5μmol/L 的Ionomycin处理10、30、45、60、120、180 min的精子AR率分别为17.2％、25.0％、41.2％、52.2％、54.0％和55.0％．结论：Ionomycin可以诱导小鼠精子的顶体反应，且顶体反应率随着浓度和诱导时间的变化而变化．