维康颗粒对亚健康疲劳小鼠不同组织氧化应激损伤的干预作用

摘要

目的:探讨氧化应激损伤与亚健康疲劳的关系及维康颗粒的干预作用.方法:将120只小鼠随机分为6组:正常组、模型组、维康颗粒高、中、低质量分数组、自然恢复组,每组20只.观察各组小鼠外观行为学改变、疲劳情况、血浆、脑、肝、骨骼肌组织中氧化应激指标的变化.结果:与模型组相比,其他5组小鼠力竭游泳时间升高,均有显著性差异(P<0.01),维康颗粒高、中、低质量分数组游泳时间高于自然恢复组(P<0.05),维康颗粒高、中质量分数组力竭游泳时间与正常组相比无显著性差异(P>0.05).与正常组相比,模型组小鼠血浆、脑、肝脏、骨骼肌内MDA升高,SOD、TAC下降(P<0.01),而维康颗粒高质量分数组无显著性差异(P>0.05).与模型组相比,自然恢复组小鼠血浆MDA、SOD、TAC均无统计学差异(P>0.05),而维康颗粒高、中、低质量分数组各项氧化应激指标均有统计学差异(P<0.05).结论:亚健康疲劳状态下小鼠存在显著地氧化应激损伤,维康颗粒可通过降低模型小鼠的氧化应激损伤,促进亚健康疲劳状态向正常状态转化.%Aim:To investigate the effects of oxidative stress on the fatigue-type sub-health state and its oxidative stress indicators after the intervention of weikang-granula.Methods:120 KMmice were ran-domly divided into 6 groups:normal control group,fatigue-type sub-health model group,weikang-granula high,medium,low mass fraction group and model of natural recovery group.The behavior changes,ex-haustive swimming time,oxidative stress indicators of all the groups were observed.Results:Compared with control group,the exhaustive swimming time of model group was markedly decreased (P <0.01), while weikang-granula intervention group was significantly increased and had no statistical difference with control group (P >0.05).Compared with the model group,the MDA,levels in plasma,liver,brain, skeletal muscle of weikang-granula intervention groups were significantly decreased while the SOD and TAC levels increased (P <0.05).The oxidative stress parameters of the natural recovery group mice showed no significant difference (P >0.05).Compared with the normal group,the MDA levels in the plasma,liver,brain,skeletal muscle of low mass fraction group and model group mice were significantly increased while the SOD and TAC levels decreased (P <0.05).The MDA levels of middle mass fraction group mice showed no significant difference (P >0.05 ),while the TAC levels decreased significantly (P <0.05).The SOD levels in Plasma of middle mass fraction group mice were decreased significantly (P <0.05),While the SOD level in liver,brain,skeletal muscle showed no significant difference (P >0.05).Conclusion:It exists oxidative stress injury in fatigue-type sub-health mice and weikang-granula can adjust anti-oxidation effect and promote them to the normal level.