He-Ne激光辐照对大豆幼苗异黄酮含量的影响

摘要

用波长为632.8 nm的He-Ne激光辐照大豆种子胚,研究辐照时间对发芽率、幼苗株高及幼苗子叶、叶片和茎中大豆异黄酮含量的影响.结果表明:大豆种子胚经3.6 mW/mm2激光辐照l-7 min后,其发芽率、幼苗株高、幼苗苯丙氨酸转氨酶(PAL)活性、游离苯丙氨酸(Phe)和大豆异黄酮含量均高于对照组(ck).其中以幼苗叶辐照5 min后和子叶辐照7 min后,大豆异黄酮含量的提高最为明显,分别为42.6%和36.7%.本研究还从大豆异黄酮合成代谢起始酶PAL活性、合成前体Phe含量两方面探讨了激光辐照大豆种子胚提高其幼苗大豆异黄酮含量的机理.%After soybean germs were exposed in a He-Ne laser (λ = 632.8 nm) illumination, its effects on germination, height of seedlings and content of soy isoflarone in seedling cotyledon, leaves and stems with exposure time were studied. The results showed that germination, height of seedlings, activity of phenylalanine ammonia-lyase (PAL), free amino acids and content of soy isoflarone were higher than those before the germs being exposed for 1, 3, 5 and 7 minutes. The optimal increasememts of soy isoflavone happened in seedling leaves (42.6％) after 5 minutes and in cotyledon (36.7％) after 7 minutes exposure, respectively. Moreover, a possible mechanism for the increase of the content of soy isoflarone was also discussed with initial enzyme activity of anabolism of soy isoflarone(PAL) and synthetic preeursor Phe content.