用CTAB法从感染草莓镶脉病毒(SVBV)的草莓叶片中提取总DNA,设计特异性引物扩增含有SVBVORF Ⅰ的片段,克隆并测序.序列分析表明,该片段全长1 033个核苷酸,其中包含的ORFⅠ全长987个核苷酸,编码328个氨基酸.结果表明:将其与花椰菜花叶病毒属其他成员的ORF Ⅰ序列相比较,中国SVBVORFⅠ与美国SVBV ORFⅠ序列相似性最高,达88.3％.进一步构建SVBV及其同属其他成员ORFⅠ的系统关系树,结果显示:中国SVBV ORF Ⅰ与美国SVBV ORFⅠ单独形成1个亚分支,说明来源于草莓的2个SVBV亲缘关系最近.%The total DNA was extracted from strawberry leaves infected with SVBV by CTAB method. Specific primer pair was designed to amplify the fragment including SVBV ORF I , and then the fragment was cloned and sequenced. Sequence analysis results showed that the fragment was compzosed of 1 033 nts, including 987 nts of full-length ORF I gene encoding 328 amino acids. Comparing the sequence of SVBV ORF I with that of the ORF I of other members of Caulimovirus, the result showed that Chinese SVBV ORF I shared the highest sequence similarity (88.3%) with that of the American SVBV. A phy-logenetic tree based on alignment of the nucleotide sequences of ORF I of SVBV and other members of Caulimovirus was constructed. The result indicated that the Chinese SVBV ORF I and the American SVBV ORF I clustered into a separate subgroup. It illustrated that the two SVBV derived from strawberry had the closest relationship.
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