芸薹链格孢菌孢子萌发及附着胞形成的初步定量研究

摘要

采用常规流行学方法研究了芸薹链格孢菌的孢子萌发及附着胞形成.结果表明:一般浸入水中2h后就有少量孢子萌发,10 h孢子萌发率可达90％以上；孢子萌发时各个细胞均可萌发形成芽管,向不同方向伸长,形成附着胞,20 h附着胞形成率可达50％以上；花粉和叶面物质对孢子萌发有促进作用；孢子抗干燥能力较弱,萌发孢子只须经4h干燥即可完全丧失存活能力；芸薹链格孢菌孢子萌发及附着胞形成的温度范围均为10～25℃,15℃为孢子萌发及附着孢形成的最适温度,温度达40℃后孢子不再萌发.%The study adopted a conventional epidemic experiment on spores germination and appressorium formation.The results showed that spores of Alternaria brassicae can germinate easily in pure water,a few spores can germinate after 2 hours of immersion,and the spores of Alternaria brassicae can germinate over 90％ after 10 hours.Each cell may germinate and form the tube,and elongate to different directions.The rate of appressorium can be over 50 ％ after 20 hours.Substance in the leaf surface and pollen can accelerate the germination of spores.The spores don't have strong ability to resist drying condition,the germinated spores completely died after 4 hours in drying condition.The temperature of the sporulation and appressorium formation were both about 10 ～ 25 ℃.It was at 15 ℃ that the ratio of spores germination and the rate of appressorium formation were the highest.The spores cannot sporulate over 40℃.