青蒿－鳖甲药对配伍对 MRL／lpr狼疮小鼠IL －6、IFN －γ分泌及狼疮性肾炎病理改变的影响

摘要

Objective:To investigate the effect of Qinghao-biejia group on auto-antibody production and pathologic change of lupus nephritis in MRL/lpr mice and the possible mechanism of qinghao-biejia group in treating systemic lupus erythematosus( SLE) .Meth-ods:IL-6 and IFN-γin serum of the MRL/lpr mice were detected by enzyme linked immunosorbent assay(ELISA).Renal tissue was stained by HE,PAS and Masson.Results:In the low and hight dose qinghao-biejia groups,as compared with the normal control group,the level of IL-6 was significantly lower( P<0.05,P<0.01);The level of IFN-γwas lower in the hight dose than in the model group(P<0.05);And renal pathological change was milder.Conclusion:qinghao-biejia group could inhibit the production of IL-6 and secretion of IFN-γand improve the pathologic lesion of lupus nephritis in MRL/lpr mice.%目的：观察青蒿－鳖甲药对对MRL／lpr狼疮模型小鼠相关免疫指标及狼疮性肾炎（ lupus nephri－tis，LN）病理改变的影响，探索青蒿－鳖甲药对对系统性红斑狼疮（ systemic lupus erythematosus，SLE）的治疗机理。方法：采用ELISA方法检测小鼠血清中的IL－6、IFN－γ含量，对肾脏组织进行HE常规染色及PAS、Masson特殊染色。结果：灌胃青篙－鳖甲药对水煎液后，低、高剂量组MRL／lpr小鼠血清中的IL－6水平显著低于正常对照组（P＜0．05或P＜0．01）；高剂量组血清中的IFN－γ水平与模型组相比，显著下降（ P＜0．05）；高剂量组肾脏组织HE、PAS、Masson染色病理改变差异显著。结论：青蒿－鳖甲药对能抑制MRL／lpr小鼠血清中IL－6的生成，减少小鼠血清中IFN－γ的分泌，能明显改善小鼠狼疮性肾炎的病理状态。