BTLA与HVEM相互作用对T细胞活化的影响

摘要

目的：探讨小鼠髓系树突状细胞（BMDC）上HVEM与T细胞上BTLA相互作用对T细胞活化的影响。方法：C57BL／6小鼠的BMDC与T细胞混合培养，HVEM抗体封闭BMDC上的HVEM后，采用MTT法检测T细胞的活化增殖，ELISA试剂盒检测IL-2的分泌。结果：混合培养的启动和早期（0～48 h）、中晚期（48～96 h）以及全程（0～96 h），加入HVEM抗体实验组的T细胞增殖效应均明显高于对照组（P＜0．05），同时CD3＋T细胞组和CD4＋T细胞组IL-2的分泌明显高于对照组（P＜0．05）。结论：BTLA-HVEM相互作用产生的负性信号对T细胞的活化起抑制作用，特别是在BMDC与T细胞混合培养的启动和早期（0～48 h），BTLA负性信号就有抑制T细胞活化的作用，有别于传统的负反馈调节抑制，提示T细胞的活化可能存在多种负性调节模式。%Objective:To investigate the effect of interaction between B and T lymphocyte attenuator (BTLA)and HVEM of mouse bone marrow-derived dendritic cells(BMDC)on T cell activation.Methods:T cells and BMDC from C57BL/6 mouse were mixed and cultured.After blocking HVEMon BMDC by an-tibodies against HVEM,MTT method was used to examine T cell proliferation and the secretion of IL-2 was tested by ELISA kit.Results:In the priming and early phase (0-48 h),or middle and later phase (48-96 h),or all phase (0-96 h)of mixing culture,the proliferation of T cells from experimental groups with addition of HVEMantibodies was higher compared with control groups (P<0.05),and the secretion of IL-2 from CD3 +T cell and CD4 +T cell groups was also higher compared with control groups (P<0.05 ). Conclusion:The negative signal produced by interaction between BTLA and HVEM played the inhibition role in T cell activation.Especially in the priming and early phase (0-48 h)of mixing culture of T cells and BMDC,the negative signal from BTLA already played such inhibition role,exhibiting another negative regulation mode different from traditional negative feedback regulation mode and suggesting the existence of multiple negative regulation for T cell activation.