Biodistributions of micro-PET tracer 1 8 F-FDG,1 8 F-RGD,and 1 8 F-FET in the LN229 induced glioma nude mice were performed using micro-PET technology.Tyro-sine and NOTA modified [c(RGDyK)]2 were employed as precursors for the synthesis of 1 8 F-FET and 1 8 F-RGD.Glioma model was established via intracerebral injection of LN229 cells and the biodistribution in tumor and major organs at 30 min and 60 min postinjection of 1 8 F-FDG,1 8 F-RGD,and 1 8 F-FET were determined by Gamma counting, respectively.The results showed more than 98% purity for 1 8 F-FET and 1 8 F-RGD. After 60 min injection of three radiotracers,the uptake values of 1 8 F-FDG,1 8 F-RGD, and 1 8 F-FET in tumor and brain were 4.89±0.65%ID/g ((2.72±0.76)%ID/g),2.10± 0.32%ID/g ((0.23±0.01 )%ID/g),and 3.02 ±0.64%ID/g ((0.74 ±0.12)%ID/g), respectively.In addition,the ratio of tumor to brain of 1 8 F-FDG,1 8 F-RGD,and 1 8 F-FET were determined as 0.64 ± 0.07 (1.80 ± 0.32 )、8.22 ± 1.03 (9.13 ± 1.21 )and 2.15± 0.48 (4.08 ± 0.92 ).Micro-PET imaging also represented a clearly visualized tumor circumscription.In conclusion,micro-PET combined with 1 8 F-FET and 1 8 F-RGD could be a promising technique for the diagnosis and research of glioma.%为研究肿瘤显像剂18 F-FDG、18 F-RGD 和18 F-FET 在 LN229脑胶质瘤模型裸鼠体内分布和Micro-PET 肿瘤显像,采用酪氨酸和 NOTA 修饰的[c(RGDyK)]2为前体分别合成18 F-FET 和18 F-RGD；颅内注射 LN229细胞建立脑胶质瘤模型,研究18 F-FDG、18 F-RGD 和18 F-FET 在注射30 min 和60 min体内脏器分布以及荷瘤鼠 Micro-PET 显像。结果表明,18 F-FET 和18 F-RGD 放化纯度大于95%。荷瘤鼠体内分布显示,注射后1 h,18 F-FDG、18 F-RGD 和18 F-FET 在脑胶质瘤和脑组织内(括号内)的摄取分别为(4.89±0.65)% ID/g((2.72±0.76)% ID/g)、(2.10±0.32)% ID/g((0.23±0.01)% ID/g)、(3.02±0.64)%ID/g((0.74±0.12)%ID/g),18 F-FDG、18 F-RGD 和18 F-FET 在肿瘤和脑摄取放射性比值分别为0.64±0.07(1.80±0.32)、8.22±1.03(9.13±1.21)和2.15±0.48(4.08±0.92),Micro-PET 肿瘤显像清晰。结果提示,18 F-FET 和18 F-RGD 更适用于脑胶质瘤的诊断。
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