基质金属蛋白酶3及其抑制剂3在子痫前期患者胎盘中的表达

摘要

To detect the expression of matrix metalloproteinases-3 (MMP-3 )and tissue inhibitor of metal-loproteinases-3 (TIMP-3 )in placenta of the patients with preeclampsia, and to investigate the role of MMP-3 and TIMP-3 in the pathogenesis of preeclampsia. Methods:The levels of MMP-3 and TIMP-3 in placental tissues were measured from 34 patients with preeclampsia (study group)and 32 normal pregnant women (control group)by immunohistochemistry. HE staining was used in order to count the number of the vessles. Results:MMP-3 and TIMP-3 were expressed in the decidual cell and the villous trophoblast in both group. Whether in decidual cell or in villi trophoblast cell, MMP-3 in preeclampsia group was significantly lower than that in the control group, but TIMP-3 was obviously higher in preeclampsia group. The count of vessels reduced in preeclampsia group. Conclusions:The differences of MMP-3 and TIMP-3 in placental tissues between preeclampsia group and control group suggest that those changes may associate with the pathogenesis of preeclampsia.%目的:通过检测子痫前期患者胎盘组织中基质金属蛋白酶3(MMP-3)及其抑制剂3(TIMP-3)的表达,以探讨两者的关系及其在子痫前期发病中的作用.方法:收集34例子痫前期患者(子痫前期组)及32例正常晚期妊娠妇女(正常对照组)的胎盘组织,用免疫组化法检测MMP-3及TIMP-3在胎盘组织中的表达.同时进行HE染色,选取5个高倍视野,计数绒毛血管数.结果:①MMP-3和TIMP-3在胎盘组织的蜕膜细胞和绒毛滋养细胞均有表达.②子痫前期组胎盘蜕膜细胞和绒毛滋养细胞中MMP-3的表达均显著低于对照组(P＜0.01).TIMP-3在子痫前期组胎盘蜕膜细胞和绒毛滋养细胞中的表达量均显著高于对照组(P＜0.01).③子痫前期组胎盘绒毛血管密度明显低于对照组(p＜0.01).结论:子痫前期患者胎盘中MMP-3与TIMP-3的表达情况与正常晚期妊娠妇女存在差异,提示妊娠妇女胎盘组织中MMP-3和TIMP-3的表达水平变化可能与子痫前期的发生、发展相关.