建立了固相萃取/高效液相色谱( SPE/HPLC)测定化妆品中5种抗组胺药物(多西拉敏、曲吡那敏、溴苯那敏、苯海拉明、氯苯沙明)残留的分析方法.试样经三氯乙酸溶液超声提取,PCX柱净化后,以甲醇-磷酸盐缓冲溶液为流动相,经C18柱分离后进行HPLC检测.5种抗组胺药物在5.0～ 100 mg/L范围内均呈良好的线性关系,线性系数均大于0.999.在5.0、10.0、25.0 mg/kg 3个加标水平下的平均回收率为92％～ 108％,相对标准偏差(RSD,n=6)为2.1％～4.2％,检出限为0.5～1.0 mg/L.该方法灵敏度高、重现性好、定量准确.%A solid phase extraction coupled with high performance liquid chromatography ( SPE/ HPLC) was developed for the determination of five antihistamines, e. g. doxylamine, tripelen-namine, brompheniramine, diphenhydramine, chlorphenoxamine, in cosmetics. The samples were extracted with trichloroacetic acid solution under ultrasonication, and cleaned up with PCX solid phase extraction. The separation of five antihistamines was carried out on a C18 column using metha-nol - phosphate buffer as mobile phase. The calibration curves of five drugs were linear in the range of 5. 0 - 100 mg/L, with correlation coefficients more than 0. 999. The mean recoveries at spiked levels of 5.0, 10.0, 25. 0 mg/kg were in the range of 92% -108% with RSDs(n =6)of 2. 1% -4. 2% . The limits of detection were in the range of 0. 5 -1.0 mg/L. The method was sensitive, reproducible and accurate.
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