自动净化及新型酶联免疫法测定废气中的二噁英

摘要

研究了一种适用于二噁英生物检测法的样品前处理仪器(SPD-600),考察了不同条件下的净化效果和内标回收率,建立了一种新型酶联免疫系统(DXS-600)测定二噁英的方法.研究表明,SPD-600在60℃加热的条件下净化效果最好,杂质基本被去除,内标回收率与人工制备的多层硅胶柱净化的回收率基本相当；DXS-600采用平衡除外的方法,避免了常规酶联免疫方法中的竞争步骤,消除了实验过程中的人为不确定因素,从而提高了灵敏度和稳定性；采用SPD-600净化、DXS-600测定的组合分析了21个废弃物焚烧废气样品,并与高分辨气相色谱-高分辨质谱的测定结果进行比较.结果表明,两种方法测定结果之间具有很高的相关性(r2 =0.985 8).SPD-600净化结合DXS-600测定方法可作为二噁英的快速筛查方法.%The application of a pretreatment instrument, SPD - 600, in dioxin-like substances determination by a new enzyme-linked immunosorbent assay method was introduced in this paper, Its clean-up efficiencies and recoveries under different conditions were evaluated. The results indicated that SPD - 600 achieved the optimum performance under a heated condition of 60℃, when almost all impurities were removed. And the recoveries of the labeled internal standards are comparable with that of the multilayer silica column treatment. In addition, an ELISA-based kinetic exclusion assay method using an automatic analysis instrument(DXS -600) was developed for dioxin analysis, which had avoided the competitive step. The results showed that DXS - 600 eliminated the artificial effects and was more sensitive and stable than the traditional ELISA. Finally, 21 flue gas samples were determined using SPD -600 combined with DXS -600 systems, and the results obtained were compared with those of the HRGC - HRMS analysis. A good correlation with r2 of 0. 985 8 was found between two methods. Therefore, the method of SPD -600 clean-up combined with DXS -600 analysis canrnbe used as a quick screening method for the determination of dioxin.