高效液相色谱法同时测定化妆品中14种禁用着色剂

摘要

A high performance liquid chromatographic( HPLC)methord was developed for the simul-taneous determination of 14 forbidden colorants( Metanil Yellow,Solvent Blue 35,Solvent Red 49, Acid Violet 49,Tetramethylpararosaniline Chloride( TMPC),Pigment Orange 5,Pigment Red 53, Pentamethyltriphenylmethane( PMTPE ), Sudan Red Ⅱ, Hexamethylenepararosaniline Chloride (HMPC),Solvent Red 24,Rhodamine B,Disperse Yellow 3,Sudan Red Ⅰ)in cosmetics. Cos-metic samples were extracted and dispersed with tetrahydrofuran,and some matrixes were precipitated out after additon of ammonium acetate solution and methanol. After centrifuged and filtered,the ex-tract was separated on an Agilent poroshell 120 EC-C18 column(2. 7 μm,3. 0 mmí100 mm)by a gradient elution with 10 mmol/L ammonium acetate-acetonitrile as mobile phase. Detection wave-lengths were set at 416,514,590 nm,respectively. The qualitative analysis was based on chroma-tographic retention time and UV spectrum,and the quantitative analysis was performed by the stand-ard curve method of peak area. The linear ranges of 14 analytes were in the range of 0. 2-20 μg/mL,with correlation coefficients more than 0. 999. The limits of quantitation( LOQs)were in the range of 3-10 μg/g. The average recoveries at three spiked levels ranged from 91. 3% to 110. 9%with RSDs less than 10% . This method was accurate,simple,sensitive and reliable,and could be used for the analysis of 14 forbidden colorants in cosmetics.%采用高效液相色谱建立了同时测定化妆品中14种禁用着色剂（吖啶黄、溶剂蓝35、溶剂红49、酸性紫49、氯化四甲基副玫瑰苯胺、颜料橙5、颜料红53、氯化五甲基副玫瑰苯胺、苏丹红Ⅱ、氯化六甲基副玫瑰苯胺、苏丹红Ⅳ、罗丹明B、分散黄3、苏丹红Ⅰ）的分析方法。待测化妆品样品经四氢呋喃分散，并对目标物进行提取后，用含有醋酸铵的甲醇-水混合溶液将基质析出，提取液经离心过滤后，以10 mmol/L的醋酸铵溶液-乙腈作为流动相在Agilent poroshell 120 EC-C18（2.7μm，3.0 mm×100 mm）色谱柱上梯度洗脱进行分离，检测波长为416，514，590 nm。以目标物的色谱保留时间和紫外光谱图进行定性，以色谱峰的峰面积用标准曲线外标法进行定量。在优化条件下，各目标物的线性范围为0.2~20μg/mL，相关系数均大于0.999。14种禁用着色剂的定量下限为3~10μg/g。在低、中、高3个加标水平下，各目标物的回收率为91.3％~110.9％，相对标准偏差（ RSD）均低于10％。方法准确、简便、灵敏、可靠，可用于化妆品中此14种禁用着色剂的定量测定。