液相色谱-质谱联用接口技术的研制及性能表征

摘要

A homemade LC-MS interface was developed.The interface included atmospheric interface,ion source and electronic communication interface which connected to high-performance liquid chromatography and mass spectrometry's software.The team self-developed API-TOFMS 10000 was successfully linked to Thermo Fisher Scientific Diana U3000.Experimental results showed that the self-built LC-MS was operated with a good stability (RSD =5.6％),the linear correlation coefficient (r2) was 0.995 8,the detection range:2-2 000 pg,the limit of quantitation:2 pg,and the detection limit:0.5 pg.Piroxicam,theophylline and prednisone could be obviously detected.The experimental data showed that the self-developed LC-MS interface could meet the testing requirements,and the serf-built LC-MS has an application potential in biochemistry,pharmaceutical analysis,food safety,environmental detection etc.%自制了一套液相色谱-质谱联用接口,接口包含大气压接口、离子源及连接高效液相色谱进样器和质谱采集软件的电子通讯接口.成功地将赛默飞世尔高效液相色谱戴安U3000与团队自主研制的API-TOFMS10000相连.实验结果表明,自行搭建的液质联用仪有很好的稳定性(RSD=5.6％),线性相关系数(r2)为0.995 8,线性范围为2～2000 pg,定量下限为2 pg,检出限为0.5 pg.可明显检出吡罗昔康、茶碱和泼尼松3种标准药品.实验数据表明自行研发的液相色谱-质谱联用接口满足应用要求,搭建的液相色谱-质谱联用仪在生物化学、医药分析、食品安全、环境检测等领域具有应用潜力.