激光解吸电离傅立叶变换离子回旋共振质谱用于食用油的快速分析

摘要

A fast and direct method was established for the qualitative analysis of triacylglycerols (TAGs) in edible oils using laser desorption ionization Fourier transform ion cyclotron resonance mass spectrometry(LDI-FTICR-MS).In the conditions of 45％ laser power,100 Hz laser frequency and 100 laser shots,stable and repeatable signals with relative standard deviations(RSDs) less than 10％ were obtained via this method.Using MS results and fragmented information,different kinds of edible oils could be directly distinguished from each other.34 different kinds of edible oils were clearly classified by principal component analysis(PCA) and clustering analysis at a confidence level of 95％.5％ canola doped in olive could be identified directly using this method.In addition,according to the slopes of linear curves,which kinds of oil was doped in olive oil could be preliminarily predicted.These results indicated that LDI-FTICR-MS had the potential for rapid analyzing and screening edible oils.%利用激光解吸电离傅立叶变换离子回旋共振质谱(Laser desorption ionization,LDI-FTICR-MS)建立了一种快速分析食用油中甘油三酯(TAG)的方法.在激光能量为45％,激光频率100 Hz和辐照次数100shots的条件下,可以获得稳定重复的信号(RSD＜ 10％).通过TAG的一级质谱图和二级碎片信息可以初步区别不同类型的食用油.在置信度为95％条件下,利用主成分分析法和聚类分析法可以有效地将34种食用油归类.此外,利用该方法可直接识别橄榄油中掺杂5％的菜籽油且根据线性公式可初步预测橄榄油中掺杂油品的种类.分析数据表明,LDI-FTICR-MS技术具有快速筛查和识别食用油的潜力.