节旋藻（Arthrospira）3个样品镍铁氢化酶 hoxY基因的克隆与测序

摘要

In this paper, the hoxY gene of hydrogenase in Arthrospira were cloned and sequenced, which Arthrospira platensis from Alkaline Lakes in the Ordos Plateau, A. platensis from Lake Chad in Africa and A. maxima from Lake Texcoco in Mexico. And their homology and genetic relationship were also analysed, in the hope of providing elementary data and theoretical bases for the research of diversity of Arthrospira from Alkaline Lakes in the Ordos Plateau. The results showed that the partial sequence of hoxY gene in Arthrospira contained 479 bp. There was a high degree of sequence homology between A. platensis from Ordos and A. platensis from Lake Chad, A. maxima which were 99. 5% and 99. 9 % respectively. The genetic relationship between A. platensis and A. maxima were closer than that from A. platensis from Ordos. Conclusion:The gene hox Y had a high conservation, in which sequence of the different samples among the same genera had low variation. However, gene sequence of the same species of Arthrospira which grew in different environments has variation.%目的：为鄂尔多斯高原碱湖节旋藻的多样性研究提供基础资料和理论依据。方法：本文对鄂尔多斯高原碱湖钝顶节旋藻( Arthrospira platensis)、乍得湖钝顶节旋藻( A. platensis)及极大节旋藻( A. maxima)的镍铁氢化酶小亚基hoxY基因进行了克隆与测序，并进行同源性及亲缘关系的分析。结果：3个节旋藻样品hoxY基因部分序列长均为479 bp。鄂尔多斯高原碱湖钝顶节旋藻 hoxY基因与不同来源的钝顶节旋藻的同源性高达99.5%，与极大节旋藻的同源性达99.9%。 A. maxima与非洲乍得湖A. platensis间的亲缘关系更近，而鄂尔多斯高原碱湖钝顶节旋藻与乍得湖钝顶节旋藻的亲缘关系相对远。结论：hoxY基因保守性高，属内不同样品间序列变化很小。同种不同来源的钝顶节旋藻由于不同环境条件等因素的影响导致基因序列上碱基发生一定的差异。