TGF-β1在宫颈组织及宫颈鳞癌盆腔淋巴结中的表达及意义

摘要

目的:通过检测TGF-β1在宫颈上皮内瘤变及ⅠA2~ⅡA宫颈鳞癌及盆腔淋巴结中的表达,探讨TGF-β1与宫颈鳞癌发生、发展及盆腔淋巴结微转移的相关性.方法:采用免疫组化S-P法检测TGF-β1在20例正常宫颈鳞状上皮组织、70例宫颈上皮内瘤变组织、100例宫颈鳞癌组织中的表达情况,同时分析其在肿瘤的不同临床阶段病理特征之间的关系.并检测TGF-β1在宫颈鳞癌相应盆腔淋巴结HE染色无转移中的表达.结果:TGF-β1蛋白在正常宫颈、CIN和宫颈鳞癌组织中表达强度逐渐增强,三者之间比较具有显著性差异(P<0.05),且TGF-β1在宫颈鳞癌中的表达强度与宫颈鳞癌的病理分级、有无淋巴结转移、浸润深度都密切相关(P<0.05).TGF-β1在宫颈鳞癌原发灶中的阳性表达与在盆腔淋巴结HE染色无转移中的阳性表达呈正相关(P<0.05).术后随访所有HE染色无盆腔淋巴结转移病人中,TGF-β1阳性表达的病人2a内复发率及3a内复发率明显高于TGF-β1阴性表达的病人,具有显著性差异(P<0.05),而1a内复发率无明显差异.结论:在宫颈鳞癌的发生发展过程及其转移方面,TGF-β1可能发挥了一定的作用,其表达与宫颈鳞癌的病理分级、转移有关,可能有望作为判断宫颈鳞癌恶性程度、有无淋巴结微转移的一项指标,并能更好的判断预后,指导治疗.%Objective:To detection the expression of TGF-β1 in cervical intraepithelial neoplasia、cervical squamous cell carcinoma and the clinical significance and pelvic lymph node micrometastases, and investigate TGF-β1 and the occurrence and development of squamous cell carcinoma of the cervix and pelvic lymph node micrometastases. Methods:The immunohistochemical method was used to detect the expression of TGF-β1 in 70 cases of cervical intraepithelial neoplasia、100 cases of cervical squamous cell carcinoma and 20 cases of normal cervical epithelium tissue paraffin sections, and investigate the relationship in different pathological character. The expression of TGF-β1had a positive correlation with that in the lymph-node. Result:The expression levels of TGF-β1and increased with increasing severity of cervical disease ( P<0 . 05 ) , TGF-β1 had closely relative to pathological differentiation、infiltration and lymph-node metastases of CSCC ( P<0 . 05 ) . The positive expression in cervical squamous cell carcinoma was positively correlated with the positive expression in negative pelvic lymph node(P<0. 05). Postoperative follow-up of all patients with pelvic lymph node metasta sis without HE staining. TGF-β1positive expression in patients with recurrence within 2 years and 3 yearsrecurrence rate was significantly higher than TGF-β1negative patients. But there was no significant difference in recurrence rate within 1 year. Conclusion:TGF-β1may play a role in the occurrence and development of cervical squamous cell carcinoma and its transfer. they are expected as an indicator to determine the degree of malignancy of CSCC. And can better determine the prognosis, guiding treatment.