咳喘宁对RSV诱发哮喘大鼠血清IL-4、IFN-γ的调节作用及对支气管平滑肌增殖活性的影响

摘要

Objective To observe the regulating effect of Kechuan Ning on serum IL-4, IFN and the proliferation of airway smooth muscle cells (ASMCs) level (OD level) in RSV induced asthma rats. And to provide experimental basis for the prevention and treatment of asthma. Methods The 60 rats were randomly divided into 6 groups. Except the normal group, the other rats were induced asthma by sensitization and virus to making models. On the day of succesful modeling, the IL-4 and IFN-γ were determined by ELISA. The smooth muscle cells were cultured and the smooth muscle cells were identified as smooth muscle cells by inverted microscope. The containing serum groups were prepared and added ASMCs at 3-7 passages. The proliferation of ASMCs (OD value was determined by MTT method. Results The ELISA result shows that the serum levels of IL-4 and IFN-γ levels in the Western medicine group and Kechuanning group were with statistical significance comparing with the model group (P<0.05). Compared with high dose and low dose groups, the serum levels of IL-4 and IFN-γlevels had statistic significance (P<0.05). MTT assay shows that the rest of five groups had statistical significance comparing the blank control group (P<0.01). Compared with the large-dose and small-dose serum groups, the difference in medium-dose serum group was significant statistically (P<0.05). Conclusion Kechuanning shows obviuos effect on treatment of RSV induced asthma rats. The airway inflammation is reduced by upregulating IFN-γ level and reducing IL-4 level expression, the body immune system is regulated by maintaining the balance of Th1/Th2. Kechuanning in treatment of bronchial asthma may be closely related to the inhibition of airway smooth muscle proliferation and influence of airway remodeling.%目的：观察咳喘宁对RSV诱发哮喘大鼠血清IL-4、IFN-γ的调节作用及对气道平滑肌细胞(airway smooth muscle cells,ASMCs)增殖水平（OD值）的影响。方法将60只大鼠随机分为6组，除正常组外，其余大鼠通过致敏、诱喘、病毒激发哮喘进行造模。造模完成当天分组干预,并用ELISA进行IL-4、IFN-γ水平的测定。造模成功后取支气管平滑肌进行细胞培养，倒置显微镜下化学荧光法鉴定为平滑肌细胞。制备大鼠不同组含药血清并加入第3-7代ASMCs中干预。运用MTT法检测各组ASMCs增殖水平（OD值）。结果 ELISA法结果，与模型组相比，西药治疗组、咳喘宁各剂量组血清IL-4、IFN-γ水平差异具有统计学意义（P<0.05）；与咳喘宁大、小剂量组相比，中剂量组血清IL-4、IFN-γ水平差异有统计学意义（P<0.05）。MTT法结果，与空白对照组比较，其余5组差异均有显著统计学意义（P<0.01）；与咳喘宁大、小剂量血清中比较，中剂量血清组差异有统计学意义（P<0.05）。结论咳喘宁对治疗RSV诱发的哮喘大鼠疗效较为显著，通过上调IFN-r水平、降低IL-4水平表达从而降低气道炎症反应，维持Th1/Th2平衡来调节机体免疫机制。咳喘宁治疗支气管哮喘可能与其能够抑制气道平滑肌增殖、影响哮喘气道重塑关系密切。