中药脑泰方对大鼠脑缺血后VEGF和Angiopoietins表达的实验研究

摘要

目的 探讨中药脑泰方对大鼠脑缺血后血内皮生长因子(vascular endothelial growth factor,VEGF)和血管生成素(angiopoietins,Ang)的表达变化及其在血管生成中的作用.方法 选择40只雄性SD大鼠,采用随机区组法分为A正常对照组,B假手术组,C MCAO模型组,D脑泰方组;,观察各组大鼠神经功能缺失计分、脑梗死体积;RT-PCR、Western blot方法检测不同组大鼠脑组织中VEGF、Ang-1及Ang-2 mRNA和蛋白的表达变化.结果 神经功能缺失计分及TTC染色显示脑泰方组脑缺血情况缓解;大鼠脑缺血后VEGF、Ang-2 的表达水平增高,脑泰方组表达显著高于模型组(P<0.05);Ang-1的表达水平在大鼠脑缺血后呈下降趋势,脑泰方组表达显著低于模型组(P<0.05).结论 大鼠脑缺血后VEGF、Ang-1和Ang-2共同协调作用促进血管生成,中药脑泰方在脑缺血损伤后治疗血管新生样作用中发挥着促进作用.%Objective To investigate Naotaifang on expression of VEGF and angiopoietins in rats after cerebral ischemia, and its roles in angiogenesis. Methods 40 male SD rats were randomly divided into the control group (A), sham-operation group (B),MCAO model group (C),Naotaifang (NTF) group (D). The score of neurological deficiency and volume of cerebral infarction were observed. The protein expression of VEGF, Ang-1 and Ang -2 in cerebral tissues of rats were detected by RT-PCR and Western blot. Results The score of neurological evaluation and TCC staining showed that the cerebral ischemia situation of NTF group have relieved. The expression of TVEGF and Ang-2 were markedly increased, the NTF group is significantly higher than the MCAO group (P<0.05). The expression of Ang-1 in rats after cerebral ischemia was markedly reduced, the NTE group is significantly lower than the model group (P<0.05). Conclusion The coordinating role of VEGF, Ang-1 and Ang-2 expression stimulate angiogenesis after cerebral ischemia. Naotaifang can promote angiogenesis in rats after cerebral ischemia-reperfusion injury.