丹栀逍遥散对多囊卵巢大鼠血清T、AMH及卵巢局部AMH蛋白表达的影响

摘要

Obejective To investigate the effect of Danzhi Xiaoyao powder on the experssion of serum testosterone (T),anti-mulerian hormone (AMH) and local ovarian AMH in polycystic ovaries (PCO) rats.Methods The PCO rat models were built by subcutaneous injection of dehydroepiandrostexone at back of SD female rats.The succesfully modeling rats were randomly devided into normal group,Diane-35 group,Danzhi Xiaoyao powder group and the blank group was also assigned.The rats were given intragastric gavage with distilled water,Diane-35 and Danzhi Xiaoyao powder,respectively,continuously for 21 days,stopped for 7 days as a cycle.After 3 cycles of administration,the rats were executed.The serum level of T and AMH were measured with radioimmunoassy.The local ovarian expression of AMH were measured with immunohistomchemical.Results Compared with the model group,the serum of T,AMH and the expression of AMH in the Danzhi Xiaoyao Powder group were reduced (P＜0.01 or P＜0.05).Conclusion Danzhi Xiaoyao powder could treat hyperandrogenism (HA) by lowering the serum T,AMH and the local ovarian AMH expression,so it can improve HA with benign regnaltion on AMH.%目的 探讨丹栀逍遥散对多囊卵巢(polycystic ovaries,PCO)大鼠血清睾酮(testosterone,T)、抗苗勒管激素(anti-mulerian hormone,AMH)及卵巢局部AMH蛋白表达的影响.方法 SD雌性大鼠以颈背部皮下注射脱氢表雄酮(dehydroepiandrostexone,DHEA)溶液构建PCO大鼠模型,判定造模成功后再随机分成模型组、达英-35组、丹栀逍遥散组,并设空白组,分别用蒸馏水、达英-35及丹栀逍遥散对大鼠灌胃给药,连续灌胃21 d,停药7d为一疗程,灌胃3个疗程后处死大鼠,采用放射免疫法测定T、AMH,免疫组织化学法检测PCO大鼠卵巢局部AMH蛋白表达.结果 与模型组比较,丹栀逍遥散组血清T、AMH水平及AMH蛋白表达强度均下降(P＜0.01或P＜0.05).结论 丹栀逍遥散治疗高雄激素血症(hyperandrogenism,HA)可能是通过降低PCO大鼠血清T、AMH水平,降低卵巢局部AMH蛋白表达强度,对AMH进行良性调节,从而改善HA.