心痛方对急性心肌梗死大鼠心肌组织内游离钙离子浓度及RYR2、PLB mRNA表达的影响

摘要

目的 观察心痛方对急性心肌梗死(acute myocardial infarction,AMI)大鼠心肌组织内游离钙离子浓度(Ca2+)及RYR2、PLB mRNA表达的影响,探讨钙稳态调节在AMI中的意义及心痛方的疗效机制.方法 50只SD大鼠分为假手术组、模型组、心痛方高剂量组、心痛方低剂量组、硫氮卓酮组.采用改进的冠脉结扎法制备AMI模型,运用激光扫描共聚显微镜法检测心肌组织内Ca2+,HE染色法测定心肌梗死面积,RT-PCR法测定心肌组织内RYR2、PLBmRNA表达.结果 各药物干预组、模型组与假手术组比较,Ca2+均增高,RYR2、PLBmRNA表达均降低,差异均具有统计学意义(P<0.01);各药物干预组与模型组比较Ca2+均降低,心梗面积均缩小,RYR2、PLBmRNA表达均增高(P<0.01);两组中药组与硫氮卓酮组比较,Ca2+均降低,心梗面积均缩小,RYR2、PLBmRNA表达均增高(P<0.01或P<0.05);中药高剂量组与中药低剂量组比较,Ca2+降低,心梗面积缩小,RYR2、PLBmRNA表达增高(P<0.01或P<0.05).结论 心痛方能缩小AMI大鼠心梗面积,且较硫氮卓酮组更有优势;心痛方可降低Ca2+并提高RYR2、PLB mRNA的表达,调节钙稳态,其对AMI大鼠的保护作用可能与减少钙超载对心肌细胞的损害,增强心肌收缩力有关.%Objective To observe the effect of Xintong Fang on intracellular free calci um concentration (Ca2+), and RYR2 and PLB mRNA expression in cardiomyocytes of acute myocardial infarction (AMI) rats, and to explore the significance of calcium homeostasis in AMI and the therapeutic mechanism of Xintong Fang. Methods Fifty SD rats were divided into the sham operation group, model group, high dose of Xintong Fang group, low dose of Xintong Fang group, diltiazem group. The AMI model was made by improved coronary ligation, and the Ca2 +in myocardium was detected by laser scanning copolymerization microscope, and the infarct size was measured by HE staining, and the expression of RYR2 and PLB mRNA in myocardium was measured by RT-PCR. Results Compared with the sham operation group, Ca2+in the drug intervention groups and model group increased, the expression of RYR2 and PLB mRNA decreased, the difference was statistically significant (P<0.01). Compared with the model group, myocardial infarction area and Ca2+in each drug intervention groups decreased, the expression of RYR2 and PLB mRNA expression increased (P<0.01). Compared with the diltiazem group, the myocardial infarction area and Ca2+in Xintong Fang groups reduced, RYR2 and PLB mRNA expression increased (P<0.01 or P<0.05). Compared with the low-dose of Xintong Fang group, the myocardial infarction area and Ca2+in the high-dose of Xintong Fang group reduced, the expression of PYR2 and PLB mRNA increased (P<0.01 or P<0.05). Conclusion Xintong Fang can reduce myocardial infarction area in rats with acute myocardial infarction, and has more advantages than sulfiazem group. Xintong Fang could decrease the concentration of Ca2+, increase the expression of RYR2 and PLB mRNA, and regulate calcium homeostasis. Its protective effect on AMI rats may be related to reduced myocardial damage of calcium overload and enhanced.