通过室外人工接虫，研究了表达Cry1A 杀虫蛋白的中嘉8号杨树转 Bt 植株对杨扇舟蛾的田间抗性和对非靶标生物的影响。连续3年的试验结果表明，人工接虫1周后，转 Bt 植株有虫株率为4％～12％，平均为8％；对照组未转化植株有虫株率都在90％以上，平均为93．3％。接虫2个月后，转Bt 植株虫口密度为2．1～3．1头/株，平均为2．5头/株；对照组未转化植株虫口密度为9．7～12．3头/株，平均为11．2头/株，并且转Bt植株和对照组未转化植株林间生物丰度差异都不显著。初步认定，中嘉8号杨树转 Bt 植株对靶标害虫-杨扇舟蛾幼虫具有明显的抗性，而对非靶标生物无明显的毒害作用，具有进一步大田释放的价值。%The transgenic popular expressing BtCry1A toxin were conducted for resistance and influence of non-target organisms to the Clostera anachoreta by artificial inoculation in the field .The three years'research results showed that attacking rate of the transgenic poplar were 4%~12% and those of non-Bt poplar were more than 90% after inoculating 1 week, the average respectively 8% and 93.3%.after inoculating 2 months population density of the transgenic poplar were 2.1~3.1 insects per plant those of non -Bt poplar are 9.7 ~12.3 insects per plant, the average were 2.5 and11.2 insects per plant. In addition,biological abundance differences were not significant between the transgenic plant and non -Bt plant.It was suggested that the transgenic Bt poplar had the obvious resistances to C .anachoreta larvae and no distinct toxicity to non-target organisms,which was of great value to further release to the field .
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