Three SNaPshot multiplex assays were developed to test 23 coding region single nucleotide polymorphisms(SNPs) and one control region SNP outside hypervariable regions(HVR)Ⅰand Ⅱ,which was aimed at increasing the discrimination power of the mitochondrial DNA(mtDNA) typing in forensic casework,and confirming haplogroup assignments of mtDNA profiles in both human population studies and medical research.The selected SNPs targeted the East Asian phylogeny.These multiplex assays were validated by comparing with the sequencing analysis of samples chosen randomly.The mtDNA variations of 100 unrelated individuals from the Wuhan population in China were examined and classified into 31 haplotypes,and the haplotype diversity was estimated to be 0.952.The multiplex SNaPshot method is rapid and robust,and suitable for large-scale screening studies of mtDNA variability.
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