Dioscin-induced Apoptosis of Human LNCaP Prostate Carcinoma Cells through Activation of Caspase-3 and Modulation of Bcl-2 Protein Family

摘要

Dioscin is a natural steroid saponin derived from several plants,showing potent anti-cancer effect against a variety of tumor cell lines.In the present study,we investigated the anti-cancer activity of dioscin against human LNCaP cells,and evaluated the possible mechanism involved in its antineoplastic action.It was found that dioscin(1,2 and 4μmol/L)could significantly inhibit the viability of LNCaP cells in a time-and concentration-dependent manner.Flow cytometry revealed that the apoptosis rate was increased after treatment of LNCaP cells with dioscin for 24 h,indicating that apoptosis was an important mechanism by which dioscin inhibited cancer.Western blotting was employed to detect the expression of caspase-3,Bcl-2 and Bax in LNCaP cells.The expression of cleaved caspase-3 was significantly increased,and meanwhile procaspase-3 was markedly decreased.The expression of anti-apoptotic protein Bcl-2 was down-regulated,whereas the pro-apoptotic protein Bax was up-regulated.Moreover,the Bcl-2/Bax ratio was drastically decreased.These results suggested that dioscin possessed potential anti-tumor activity in human LNCaP cells through the apoptosis pathway,which might be associated with caspase-3 and Bcl-2 protein family.